Author | dc.contributor.author | Moore, C. | |
Author | dc.contributor.author | Sauma Mahaluf, Daniela | es_CL |
Author | dc.contributor.author | Morales Peña, José | es_CL |
Author | dc.contributor.author | Bono Merino, María Rosa | es_CL |
Author | dc.contributor.author | Rosemblatt Silber, Mario César | es_CL |
Author | dc.contributor.author | Fierro, J. A. | es_CL |
Admission date | dc.date.accessioned | 2011-05-03T18:29:02Z | |
Available date | dc.date.available | 2011-05-03T18:29:02Z | |
Publication date | dc.date.issued | 2009-07 | |
Cita de ítem | dc.identifier.citation | TRANSPLANTATION PROCEEDINGS, Volume: 41, Issue: 6, Pages: 2670-2672, 2009 | es_CL |
Identifier | dc.identifier.issn | 0041-1345 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/119186 | |
Abstract | dc.description.abstract | CD4 CD25 Foxp3 regulatory T cells (Treg) mediate immunologic self-tolerance and
suppress immune responses. In the gut, a subset of dendritic cells is specialized to induce
Treg in a transforming growth factor- (TGF- )- and retinoic acid (RA)-dependent
manner. The aim of this study was to establish if RA synergizing with TGF- induced
antigen specific CD4 CD25high Foxp3 Treg portraying gut homing receptors. Splenic
CD4 CD25 Foxp3 naïve T cells from DO11.10 mice were cocultured with splenic
CD11c dendritic cells from Balb/c mice in the presence of TGF- , RA, and low levels of
an antigenic peptide. After 5 days of culture, cells were analyzed for the expression of
Foxp3 and the gut homing receptors CCR9 and 4 7.
The number of Foxp3 T cells generated with TGF- and RA was at least 3 times higher
than in the cultures with TGF- alone and 15 times higher than in controls without
exogenous cytokines. Also, supplementation of the cultures with RA induced the
expression of the intestinal homing receptors CCR9 and 4 7. Our results showed that
coculture of naïve T cells with antigen-presenting cells in the presence of TGF- and RA
represents a powerful approach to generate Treg with specific homing receptors. | es_CL |
Patrocinador | dc.description.sponsorship | Supported by Fondecyt grants 1060834, 1060253, 1080416,
Universidad Andres Bello grant DI-08-08/I and Clinica las Condes. | es_CL |
Lenguage | dc.language.iso | en | es_CL |
Publisher | dc.publisher | ELSEVIER SCIENCE INC | es_CL |
Keywords | dc.subject | TGF-BETA | es_CL |
Título | dc.title | Transforming Growth Factor-beta and All-Trans Retinoic Acid Generate Ex Vivo Transgenic Regulatory T Cells With Intestinal Homing Receptors | es_CL |
Document type | dc.type | Artículo de revista | |