Author | dc.contributor.author | Zhang, Ziyang | |
Author | dc.contributor.author | Ito, Wulf D. | es_CL |
Author | dc.contributor.author | Hopfner, Úrsula | es_CL |
Author | dc.contributor.author | Böhmert, Björn | es_CL |
Author | dc.contributor.author | Kremer, Mathias | es_CL |
Author | dc.contributor.author | Reckhenrich, Ann K. | es_CL |
Author | dc.contributor.author | Harder, Yves | es_CL |
Author | dc.contributor.author | Lund, Natalie | es_CL |
Author | dc.contributor.author | Kruse, Charli | es_CL |
Author | dc.contributor.author | Machens, Hans Günther | es_CL |
Author | dc.contributor.author | Egaña, José T. | es_CL |
Admission date | dc.date.accessioned | 2011-11-25T15:10:36Z | |
Available date | dc.date.available | 2011-11-25T15:10:36Z | |
Publication date | dc.date.issued | 2011 | |
Cita de ítem | dc.identifier.citation | Biomaterials 32 (2011): 4109-4117 | es_CL |
Identifier | dc.identifier.other | doi:10.1016/j.biomaterials.2011.02.036 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/119369 | |
General note | dc.description | Artículo de publicación ISI | es_CL |
Abstract | dc.description.abstract | Increasing evidence suggests that vascular resident endothelial progenitor cells (VR-EPCs) are present in
several organs, playing an important role in postnatal neovascularization. Here, we isolated and characterized
VR-EPCs from cardiac tissue in vitro, evaluating their regenerative potential in vivo. VR-EPCs showed
to be highly clonogenic and expressed several stem and differentiation markers. Under endothelial
differentiation conditions, cells form capillary-like structures, in contrast to osteogenic or adipogenic
differentiation conditionswhere no functional changes were observed. After seeding in scaffolds, cells were
distributed homogeneously and directly attached to the scaffold. Then, cell seeded scaffolds were used to
induce dermal regeneration in a nude mice full skin defect model. The presence of VR-EPCs enhanced
dermal vascularization. Histological assays showed increased vessel number (p < 0.05) and cellularization
(p < 0.05) in VR-EPCs group. In order to explore possible mechanisms of vascular regeneration, in vitro
experiments were performed. Results showed that pro-angiogenic environments increased the migration
capacity (p < 0.001) and ability to formcapillary-like structures (p < 0.05) of VR-EPC. In addition, VR-EPCs
secreted several pro-angiogenic molecules including VEGF and PDGF. These results indicate that a highly
clonogenic population of VR-EPCs might be established in vitro, representing a new source for therapeutic
vascularization in tissue engineering and regeneration. | es_CL |
Patrocinador | dc.description.sponsorship | This work was supported by grants from University Hospital
rechts der Isar, Technische Universität München To HGM; a clinic
research grant from Technische Universität München to ZZ (KKF.
No. 8744556); German Research Council (DFG) IT-13/1, IT- 13/2 and
IT-13/3 to WDI; European Regional Development Fund (ERDF) to CK
and FONDAP (Nr. 15090007) to JTE. Z.Z was supported by a scholarship
from the China Scholarship Council. The authors gratefully
acknowledge the support of the TUM’s Thematic Graduate Center /
Faculty Graduate Center Medical Life Science and Technology at
Technische Universität München. | es_CL |
Lenguage | dc.language.iso | en | es_CL |
Publisher | dc.publisher | Elsevier | es_CL |
Keywords | dc.subject | Tissue engineering | es_CL |
Título | dc.title | The role of single cell derived vascular resident endothelial progenitor cells in the enhancement of vascularization in scaffold-based skin regeneration | es_CL |
Document type | dc.type | Artículo de revista | |