| Author | dc.contributor.author | García Beltrán, Olimpo | |
| Author | dc.contributor.author | Mena, Natalia P. | es_CL |
| Author | dc.contributor.author | Pérez, Edwin G. | es_CL |
| Author | dc.contributor.author | Cassels Niven, Bruce | es_CL |
| Author | dc.contributor.author | Núñez González, Marco | es_CL |
| Author | dc.contributor.author | Werlinger, Francisca | es_CL |
| Author | dc.contributor.author | Zavala, Daniel | es_CL |
| Author | dc.contributor.author | Aliaga, Margarita E. | es_CL |
| Author | dc.contributor.author | Pavez, Paulina | es_CL |
| Admission date | dc.date.accessioned | 2012-05-15T16:50:43Z | |
| Available date | dc.date.available | 2012-05-15T16:50:43Z | |
| Publication date | dc.date.issued | 2011-10-06 | |
| Cita de ítem | dc.identifier.citation | Tetrahedron Letters 52 (2011) | es_CL |
| Identifier | dc.identifier.issn | 6606–6609 | |
| Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/119396 | |
| Abstract | dc.description.abstract | Two fluorescence probes for the detection of cysteine (Cys), glutathione (GSH) and other biothiols, such
as homocysteine (Hcy) and cysteinyl-glycine (Cys-Gly), were developed. These molecular probes are
coumarin-based derivatives containing a chalcone-like moiety that reacts with biothiols through a
Michael addition reaction, leading to strong fluorescence enhancements. The reactivity of the tested biothiols
toward both probes (ChC1 and ChC2) follows the order Cys > GSH > Hcy > Cys-Gly, ChC1 being less
reactive than ChC2. Possible interference with other amino acids was assessed. ChC1 and ChC2 display a
highly selective fluorescence enhancement with thiols, allowing these probes to be used for fluorimetric
thiol determination in SH-SY5Y cells. | es_CL |
| Patrocinador | dc.description.sponsorship | This work was supported by FONDECYT (Grants #11090115
and 1100640), and the Millennium Scientific Initiative (Grant
P05-001-F). | es_CL |
| Lenguage | dc.language.iso | en | es_CL |
| Publisher | dc.publisher | Elsevier Ltd. | es_CL |
| Keywords | dc.subject | Biothiols | es_CL |
| Título | dc.title | The development of a fluorescence turn-on sensor for cysteine, glutathione and other biothiols. A kinetic study | es_CL |
| Document type | dc.type | Artículo de revista | |
