| Author | dc.contributor.author | Reyes Parada, Miguel | |
| Author | dc.contributor.author | Scorza, Cecilia | es_CL |
| Author | dc.contributor.author | Silveira, Rodolfo | es_CL |
| Author | dc.contributor.author | Dajas, Federico | es_CL |
| Author | dc.contributor.author | Cassels Niven, Bruce | es_CL |
| Admission date | dc.date.accessioned | 2012-11-12T13:43:41Z | |
| Available date | dc.date.available | 2012-11-12T13:43:41Z | |
| Publication date | dc.date.issued | 1994-04-04 | |
| Cita de ítem | dc.identifier.citation | ure Sciences, Vol. 54, nº 25, pp. 1955-1963, 1994 | es_CL |
| Identifier | dc.identifier.issn | 0024-3205 | |
| Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/119580 | |
| Abstract | dc.description.abstract | 4-Dimethylaminophenethylamine (DMAPEA) was characterized
as an MAO substrate. This compound was unaffected by
MAO-A, while its oxidation by MAO-B was linear as a
function of both time and enzyme concentration, with Km
= 5.8 ~M and Vmax = 21.2 pmol/min/mg protein, using a
crude rat brain mitochondrial suspension as source of MAO.
Both DMAPEA and its oxidation product,
4-dimethylaminophenylacetic acid (DMAPAA) , can be detected
electrochemically at 0.85 V. The high MAO-B affinity and
selectivity of DMAPEA, together with its low oxidation
potential, make this molecule a unique tool to determine
MAO-B activity in a wide variety of tissue preparations
using HPLC-ED. | es_CL |
| Patrocinador | dc.description.sponsorship | This work was partially supported by International Program in
Chemical scienees (IPICS), Programa de Desarrollo de las Ciencias
Básicas (PEDECIBA), Latin American Network for Research on Bioactive
Natural Products (LANBIO) and Fondo Nacional de Ciencia y Tecnología
(FONDECYT grant n" 915/89). | es_CL |
| Lenguage | dc.language.iso | en | es_CL |
| Publisher | dc.publisher | Elsevier , Science Ltd. | es_CL |
| Keywords | dc.subject | MAO-B subslrate | es_CL |
| Título | dc.title | 4-DIMETHY~INOPHENETHYLAMINE, A SENSITIVE, SPECIFIC, ELECTROCHEMICALLY DETECTABLE MONOAMINE OXIDASE-B SUBSTRATE | es_CL |
| Document type | dc.type | Artículo de revista | |
