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Authordc.contributor.authorMartínez, J.
Authordc.contributor.authorMartínez de la Fuente, Josuées_CL
Authordc.contributor.authorHerrero, J.es_CL
Authordc.contributor.authorCerro, S. deles_CL
Authordc.contributor.authorLobato, E.es_CL
Authordc.contributor.authorRivero de Aguilar, J.es_CL
Authordc.contributor.authorVásquez Salfate, Rodrigoes_CL
Authordc.contributor.authorMerino, S.es_CL
Admission datedc.date.accessioned2014-01-08T18:13:52Z
Available datedc.date.available2014-01-08T18:13:52Z
Publication datedc.date.issued2009-06
Cita de ítemdc.identifier.citationParasitology (2009), 136, 713–722.en_US
Identifierdc.identifier.issn0031-1820
Identifierdc.identifier.otherDOI: 10.1017/S0031182009006118
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/119664
General notedc.descriptionArtículo de publicación ISIen_US
Abstractdc.description.abstractAvian Plasmodium and Haemoproteus parasites are easily detected by DNA analyses of infected samples but only correctly assigned to each genus by sequencing and use of a phylogenetic approach. Here, we present a restriction site to differentiate between both parasite genera avoiding the use of those analyses. Alignments of 820 sequences currently listed in GenBank encoding a particular cytochrome B region of avian Plasmodium and Haemoproteus show a shared restriction site for both genera using the endonuclease Hpy CH4III. An additional restriction site is present in Plasmodium sequences that would initially allow differentiation of both genera by differential migration of digested products on gels. Overall 9 out of 326 sequences containing both potential restriction sites do not fit to the general rule. We used this differentiation of parasite genera based on Hpy CH4III restriction sites to evaluate the efficacy of 2 sets of general primers in detecting mixed infections. To do so, we used samples from hosts infected by parasites of both genera. The use of general primers was only able to detect 25% or less of the mixed infections. Therefore, parasite DNA amplification using general primers to determine the species composition of haemosporidian infections in individual hosts is not recommended. Specific primers for each species and study area should be designed until a new method can efficiently discriminate both parasites.en_US
Patrocinadordc.description.sponsorshippartially financed by the following projects: CGL2006-14129-C02-01/BOS from the Spanish Ministry of Education and Science, FONDECYT grant 1060186, travel grants from CSIC (grants CSIC-Univ. de Chile 2003 CL0012 and 2004 CL0033), and a BBVAen_US
Lenguagedc.language.isoenen_US
Publisherdc.publisherCAMBRIDGE UNIV PRESSen_US
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
Keywordsdc.subjectavian malaria,en_US
Títulodc.titleA restriction site to differentiate Plasmodium and Haemoproteus infections in birds: on the inefficiency of general primers for detection of mixed infectionsen_US
Document typedc.typeArtículo de revista


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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile