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Authordc.contributor.authorOrell, Álvaro 
Authordc.contributor.authorRemonsellez, Francisco es_CL
Authordc.contributor.authorArancibia, Rafaela es_CL
Authordc.contributor.authorJerez Guevara, Carlos es_CL
Admission datedc.date.accessioned2014-01-30T15:02:31Z
Available datedc.date.available2014-01-30T15:02:31Z
Publication datedc.date.issued2013
Cita de ítemdc.identifier.citationArchaea Volume 2013, Article ID 289236, 16 pagesen_US
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/119740
General notedc.descriptionArtículo de publicación ISIen_US
Abstractdc.description.abstractSulfolobus metallicus is a thermoacidophilic crenarchaeon used in high-temperature bioleaching processes that is able to grow under stressing conditions such as high concentrations of heavy metals. Nevertheless, the genetic and biochemical mechanisms responsible for heavy metal resistance in S. metallicus remain uncharacterized. Proteomic analysis of S. metallicus cells exposed to 100mM Cu revealed that 18 out of 30 upregulated proteins are related to the production and conversion of energy, amino acids biosynthesis, and stress responses. Ten of these last proteins were also up-regulated in S. metallicus treated in the presence of 1mM Cd suggesting that at least in part, a common general response to these two heavy metals. The S. metallicus genome contained two complete cop gene clusters, each encoding a metallochaperone (CopM), a Cu-exporting ATPase (CopA), and a transcriptional regulator (CopT). Transcriptional expression analysis revealed that copM and copA from each cop gene cluster were cotranscribed and their transcript levels increased when S. metallicus was grown either in the presence of Cu or using chalcopyrite (CuFeS2) as oxidizable substrate. This study shows for the first time the presence of a duplicated version of the cop gene cluster in Archaea and characterizes some of the Cu and Cd resistance determinants in a thermophilic archaeon employed for industrial biomining.en_US
Lenguagedc.language.isoenen_US
Publisherdc.publisherHindawien_US
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/cl/*
Títulodc.titleMolecular Characterization of Copper and Cadmium Resistance Determinants in the Biomining Thermoacidophilic Archaeon Sulfolobus metallicusen_US
Document typedc.typeArtículo de revista


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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile