Micromolar copper modifies electrical properties and spontaneous discharges of nodose ganglion neurons in vitro
Author
dc.contributor.author
Ortíz, Fernando C.
Author
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Vergara Montecinos, Cecilia
es_CL
Author
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Alcayaga Urbina, Julio
es_CL
Admission date
dc.date.accessioned
2014-12-11T18:46:11Z
Available date
dc.date.available
2014-12-11T18:46:11Z
Publication date
dc.date.issued
2014
Cita de ítem
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Biometals (2014) 27:45–52
en_US
Identifier
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DOI 10.1007/s10534-013-9685-4
Identifier
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https://repositorio.uchile.cl/handle/2250/119810
General note
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Artículo de publicación ISI
en_US
Abstract
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Copper plays a key role in aerobic cell
physiology mainly related to mitochondrial metabolism.
This element is also present at higher than basal
levels in some central nuclei and indeed, current
evidence support copper’s role as a neuromodulator in
the central nervous system. More recent data indicate
that copper may also affect peripheral neuronal
activity, but so far, there are not detailed descriptions
of what peripheral neuronal characteristics are targeted
by copper. Here, we studied the effect of
physiological concentration of CuCl2 (lM range) on
the activity of peripheral neurons using a preparation
of nodose ganglion in vitro. By mean of conventional
intracellular recordings passive and active electrical
membrane properties were studied. Extracellular copper
modified (in a redox-independent manner) the
resting membrane potential and the input resistance of
the nodose ganglion neurons, increasing the excitability
in most of the tested neurons. These results suggest
that Cu2+ modulates the activity of nodose ganglion
neurons and support nodose ganglion in vitro preparation
as a simple model to study the subcellular
mechanisms involved in the Cu2+ effects on neuron
electrical properties.
en_US
Patrocinador
dc.description.sponsorship
Financial support by Fondo Nacional de
Desarrollo Cientı´fico y Tecnolo´gico (FONDECYT, Chile),
project 1080670 (to CV).