Author | dc.contributor.author | Urtubia, Carolina | |
Author | dc.contributor.author | Devia, Jessica | es_CL |
Author | dc.contributor.author | Castro, Álvaro | es_CL |
Author | dc.contributor.author | Zamora, Pablo | es_CL |
Author | dc.contributor.author | Aguirre, Carlos | es_CL |
Author | dc.contributor.author | Tapia, Eduardo | es_CL |
Author | dc.contributor.author | Barba, Paola | es_CL |
Author | dc.contributor.author | Dell‘Orto, Paola | es_CL |
Author | dc.contributor.author | Moynihan, Michael R. | es_CL |
Author | dc.contributor.author | Petri, César | es_CL |
Author | dc.contributor.author | Scorza, Ralph | es_CL |
Author | dc.contributor.author | Prieto, Humberto | es_CL |
Admission date | dc.date.accessioned | 2010-01-22T16:55:35Z | |
Available date | dc.date.available | 2010-01-22T16:55:35Z | |
Publication date | dc.date.issued | 2008-08 | |
Cita de ítem | dc.identifier.citation | PLANT CELL REPORTS, Volume: 27, Issue: 8, Pages: 1333-1340, 2008 | en_US |
Identifier | dc.identifier.issn | 0721-7714 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/120123 | |
Abstract | dc.description.abstract | We report Agrobacterium tumefaciens-mediated
transformation of two Prunus salicina varieties,
‘Angeleno’ and ‘Larry Anne’, using a modification of
the hypocotyl slice technique previously described for
P. domestica. Regeneration rates on thidiazuron (TDZ) and
indole-3-butyric acid (IBA) supplemented Murashige and
Skoog (MS) media reached 11% for ‘Angeleno’ and 19%
for ‘Larry Anne’ hypocotyl slices. Transformation using
Agrobacterium tumefaciens GV3101 harboring a plasmid
with the neomycin phosphotransferase II (nptII) and the
green fluorescent protein (gfp) genes produced ten independent
lines, six from ‘Angeleno’ and four from ‘Larry
Anne’, representing transformation efficiencies of 0.8 and
0.3%, respectively, relative to the initial number of hypocotyl
slices. Plants of six lines were found to produce the
transgene encoded mRNAs. DNA blotting demonstrated
the presence of transgene sequences in trees from five lines
after 18 months of growth in the greenhouse. | en_US |
Patrocinador | dc.description.sponsorship | This work has been supported by the grant
CORFO-FDI Chile LE2-005. | en_US |
Lenguage | dc.language.iso | en | en_US |
Publisher | dc.publisher | SPRINGER | en_US |
Keywords | dc.subject | Prunus | en_US |
Título | dc.title | Agrobacterium-mediated genetic transformation of Prunus salicina | en_US |
Document type | dc.type | Artículo de revista | |