AFLP analysis of the fruit fly Ceratitis capitata

Abstract

Amplified Fragment Length Polymorphism (AFLP) analysis is an efficient ADN finger printing method based on selective amplification using PCR of restriction fragments from a total digest of genomic DNA. The AFLP technique was developed primarily to reveal the differences between cultivars of plant species(1) and has been applied to several crops. AFLP analysis has identified a larger number of molecular markers than RAPDs and RFLP in soybean(2) and cotton(3), among other crops. The AFLP technique has been used to analyze genetic polymorphism of nematodes(4-5), fungi(6), corals(7), fish(8), and humans(9). One report related to AFLP analysis of arthropod species has been published(10). Polymorphism of wild populations of C. capitata has been studied using multilocus enzyme electrophoresis and RAPDs(11-13). In this study, the AFLP technique was used to detect genetic polymorphism in C. Capitata (Wiedermann) (Diptera:Tephritidae)