Immobilized enzyme reactors. Diffusion/convection, kinetics, and a comparison of packed-column and rotating bioreactors for use in continuous-flow systems

Abstract

The roles of chemical kinetics and mass transfer in two types of bioreactors (packed-column reactors and rotating disk bioreactors), used with continuous-now sample/reagent(s) processing, are discussed in detail. A normalized quantitative comparison between these types of reactors clearly shows that rotating disk reactors afford a significantly more efficient utilization of immobilized active sites and permit the effective utilization of very small amounts of biocatalysts, Glucose oxidation by dissolved oxygen, catalyzed by immobilized glucose oxidase (EC 1.1.3.4), was used as a chemical model, The H2O2 product of the enzyme-catalyzed reaction was detected at a platinum working electrode for quantitation of the data.