Author | dc.contributor.author | Rojas, Gonzalo | |
Author | dc.contributor.author | Saldías, Soledad | es_CL |
Author | dc.contributor.author | Bittner, Mauricio | es_CL |
Author | dc.contributor.author | Zaldívar San Román, María Mercedes | es_CL |
Author | dc.contributor.author | Contreras, Inés | es_CL |
Admission date | dc.date.accessioned | 2011-06-30T13:01:58Z | |
Available date | dc.date.available | 2011-06-30T13:01:58Z | |
Publication date | dc.date.issued | 2001 | |
Cita de ítem | dc.identifier.citation | FEMS Microbiology Letters 204: 123-128 | es_CL |
Identifier | dc.identifier.issn | 0378-1097 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/121356 | |
General note | dc.description | Artículo de publicación ISI | es_CL |
Abstract | dc.description.abstract | We have cloned and sequenced the rfaH gene from Salmonella enterica serovar Typhi Strain Ty2. The gene shoed a high degree of similirity to the rfaH genes from Escherichia coli K-12 and S. enterica serovar Typhimurium. A rafH mutant was constructed by site-directed mutagenegis. This mutant produced a rough lipopolysaccharide (LPS), with an incomplete core region. The defect in LPS expression that rsults from the rafH mutation was corrected by a plasmid carrying the intact gene. The plasmid-borne rafH gene also restored normal LPS synthesis in a rafH mutant of E. coli. Reverse transcription-polymerase chain reaction analyses were performed to determine the effects of various environmental conditions on the expression of rafH. The transcription of rafH showed a growth-phase-dependent regulation, with maximal expression at the late exponential phase. Other environmental conditions, such as temperature or medium osmolarity, did not affect transcription of rafH. | es_CL |
Lenguage | dc.language.iso | en | es_CL |
Publisher | dc.publisher | Elsevier Sciences B. V. | es_CL |
Keywords | dc.subject | Salmonella typhi | es_CL |
Título | dc.title | The rfaH gene, which affects lipopolysaccharide synthesis in Salmonella enterica serovar Typhi, is differentially expressed during the bacterial growth phase | es_CL |
Document type | dc.type | Artículo de revista | |