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The rfaH gene, which affects lipopolysaccharide synthesis in Salmonella enterica serovar Typhi, is differentially expressed during the bacterial growth phase

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2001
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Rojas, Gonzalo
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The rfaH gene, which affects lipopolysaccharide synthesis in Salmonella enterica serovar Typhi, is differentially expressed during the bacterial growth phase
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Author
  • Rojas, Gonzalo;
  • Saldías, Soledad;
  • Bittner, Mauricio;
  • Zaldívar San Román, María Mercedes;
  • Contreras, Inés;
Abstract
We have cloned and sequenced the rfaH gene from Salmonella enterica serovar Typhi Strain Ty2. The gene shoed a high degree of similirity to the rfaH genes from Escherichia coli K-12 and S. enterica serovar Typhimurium. A rafH mutant was constructed by site-directed mutagenegis. This mutant produced a rough lipopolysaccharide (LPS), with an incomplete core region. The defect in LPS expression that rsults from the rafH mutation was corrected by a plasmid carrying the intact gene. The plasmid-borne rafH gene also restored normal LPS synthesis in a rafH mutant of E. coli. Reverse transcription-polymerase chain reaction analyses were performed to determine the effects of various environmental conditions on the expression of rafH. The transcription of rafH showed a growth-phase-dependent regulation, with maximal expression at the late exponential phase. Other environmental conditions, such as temperature or medium osmolarity, did not affect transcription of rafH.
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URI: https://repositorio.uchile.cl/handle/2250/121356
ISSN: 0378-1097
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FEMS Microbiology Letters 204: 123-128
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