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Proacrosin/acrosin quantification as an indicator of acrosomal integrity in fresh and frozen dog spermatozoa

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2006-06
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Cortés, Constanza J.
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Proacrosin/acrosin quantification as an indicator of acrosomal integrity in fresh and frozen dog spermatozoa
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Author
  • Cortés, Constanza J.;
  • Codelia, Verónica A.;
  • Manosalva, Iris;
  • Lange, Johanna de;
  • Reyes Solovera, Mónica de los;
  • Moreno, Ricardo D.;
Abstract
The scope of the present study was to evaluate the presence and activation of proacrosin/acrosin as a tool to determine the acrosomal status of fresh and frozen/thawed dog spermatozoa. Monoclonal antibody C5F11, directed against human acrosin, cross-reacted with dog spermatozoa and labeled the acrosome of both fresh and frozen/thawed dog spermatozoa. Frozen/thawed spermatozoa had a lesser proportion of labeled spermatozoa than fresh spermatozoa (P < 0.05). When live spermatozoa were labeled with soybean trypsin inhibitor conjugated with Alexa 488 (SBTI-Alexa 488), the proportion of acrosome-labeled fresh spermatozoa was less than frozen/thawed spermatozoa (P < 0.05). By using Western blots and enzymatic activity, frozen/thawed spermatozoa had a greater proportion of active acrosin than fresh spermatozoa. In addition, beta 1,4-galactosyl-transferase (GaIT), a plasma membrane bound protein, remained attached to frozen/thawed spermatozoa. Proacrosin is activated during freezing/thawing of dog spermatozoa, and that proacrosin/acrosin may be a good indicator of acrosomal integrity of frozen/thawed spermatozoa.
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URI: https://repositorio.uchile.cl/handle/2250/122358
ISSN: 0378-4320
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ANIMAL REPRODUCTION SCIENCE Volume: 93 Issue: 1-2 Pages: 165-175 Published: JUN 2006
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