Over-expression of forkhead box P3 and its association with receptor activator of nuclear factor-k B ligand, interleukin (IL) - 17, IL-10 and transforming growth factor-b during the progression of chronic periodontitis

Abstract

Aim: T regulatory (Treg) cells have been detected in periodontitis lesions, and forkhead box P3 (Foxp3) expression has been negatively correlated to receptor activator of nuclear factor-k B ligand (RANKL). The aim of this study was to correlate T-helper type 1 (Th1), Th2, Th17 and Treg transcription factor expressions, in gingival tissues from patients undergoing active periodontal tissue destruction, with bone loss-associated cytokines. Materials and Methods: In 10 chronic periodontitis patients undergoing disease progression, the mRNA expressions of T-bet, GATA-3, Foxp3, RORC2, interleukin (IL)- 1b, IL-10, IL-17, RANKL, interferon (IFN)-g and transforming growth factor (TGF)-b1 were quantified using real-time reverse transcription-polymerase chain reaction. The levels of these markers were compared between active and inactive periodontal lesions. Results: In active periodontal lesions, Foxp3, T-bet, RANKL, IL-17, IL-1b and IFN-g were significantly over-expressed compared with inactive lesions. The expression of IFN-g was the highest within the active periodontal lesions, similar to that of TGF-b1 within the inactive ones. There was a positive correlation between RANKL and IL-17. Additionally, RANKL and IL-17 were positively correlated with RORC2, but no correlation was detected with Foxp3. Conclusions: These results lead us to speculate that Foxp31 cells that do not have a regulatory function might have a role in the pathogenesis of active periodontal lesions by down-regulating TGF-b1 and IL-10 synthesis that lead to the over-expression of Th17-associated cytokines RANKL and IL-17.