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Associations Between Matrix Metalloproteinase-8 and -14 and Myeloperoxidase in Gingival Crevicular Fluid From Subjects With Progressive Chronic Periodontitis: A Longitudinal Study

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2010-11
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Hernández Ríos, Emma
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Associations Between Matrix Metalloproteinase-8 and -14 and Myeloperoxidase in Gingival Crevicular Fluid From Subjects With Progressive Chronic Periodontitis: A Longitudinal Study
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  • Hernández Ríos, Emma;
  • Gamonal Aravena, Jorge Antonio;
  • Tervahartiala, Taina;
  • Mäntylä, Päivi;
  • Rivera Escobar, Oriana;
  • Dezerega Piwonka, Andrea;
  • Dutzan, N.;
  • Sorsa, T.;
Abstract
Background: Matrix metalloproteinase (MMP)-8 is a central mediator in chronic periodontitis. MMP-8 can be activated by the cooperative action of otherMMPs such asMMP-14, reactive oxygen species, andmicrobial proteases. The aimof this study is to associate the levels, molecular forms, isoenzyme distribution, and degree of activation of MMP-8 and -14, myeloperoxidase (MPO), and tissue inhibitor of MMP (TIMP)-1 in gingival crevicular fluid (GCF) from patients with progressive periodontitis at baseline and after periodontal therapy. Methods: In this longitudinal study, GCF samples from active (n = 25) and inactive (n = 25) sites of subjects with periodontitis were screened at baseline for GCF levels of MMP-8 by immunofluorometric assay, of MMP-14 by specific activity assay, and of MPOandTIMP-1 by enzyme-linked immunosorbent assay.MMP-8 andMPOwere alsomeasuredafterperiodontal treatment. Molecular forms were determined by immuno-Western blot analyses and subjected to densitometric scanning and statistical analyses. Results: High MMP-8 and MPO levels and a strong MPO/ MMP-8 positive correlation were found in active and inactive sites at baseline. After treatment, decreases in MPO and MMP-8 were seen, except for active sites in which MMP-8 differences were not significant (P <0.05). Conclusions: We present initial data that show that GCF levels and associations between MPO and MMP-8 are related to progression episodes and treatment responses in patients with chronic periodontitis. Our results suggest an interaction between the MPO oxidative pathway and MMP-8 activation, and this cascade might be useful as a potential biomarker for treatment outcomes.
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This study was supported by grants from theAcademy of Finland, Helsinki (1130408), the Research Foundation of the Helsinki University Central Hospital (TYH 2010208), and from the Scientific and Technologic Investigation Resource, Santiago, Chile (1090461). The authors thank Maiju Kivisto¨, secretary, and Ritva Keva, laboratory technician, Institute of Dentistry, University of Helsinki, Helsinki, Finland, for secretarial and technical help, respectively. Dr. Sorsa is an inventor of United States patents 5652223, 5736341, 5866432, and 6143476. All other authors report no conflicts of interest related to this study.
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URI: https://repositorio.uchile.cl/handle/2250/123407
DOI: doi:10.1902/jop.2010.100196
ISSN: 0022-3492
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J Periodontol. 2010 Nov;81(11):1644-52.
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