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Authordc.contributor.authorHirsch Birn, Sandra 
Authordc.contributor.authorRonco Macchiavello, Ana María es_CL
Authordc.contributor.authorGuerrero Bosagna, Carlos es_CL
Authordc.contributor.authorMaza Cave, María Pía de la es_CL
Authordc.contributor.authorLeiva Balich, Laura es_CL
Authordc.contributor.authorBarrera Acevedo, María es_CL
Authordc.contributor.authorLlanos Silva, Miguel es_CL
Authordc.contributor.authorAlliende Rodríguez, María Angélica es_CL
Authordc.contributor.authorSilva, Francisca es_CL
Authordc.contributor.authorBunout Barnett, Daniel es_CL
Admission datedc.date.accessioned2010-01-26T18:43:07Z
Available datedc.date.available2010-01-26T18:43:07Z
Publication datedc.date.issued2008-12
Cita de ítemdc.identifier.citationNUTRITION Volume: 24 Issue: 11-12 Pages: 1103-1109 Published: NOV-DEC 2008en_US
Identifierdc.identifier.issn0899-9007
Identifierdc.identifier.other10.1016/j.nut.2008.05.018
Identifierdc.identifier.urihttp://repositorio.uchile.cl/handle/2250/123943
Abstractdc.description.abstractObjective: We assessed the impact of high serum folate concentration oil erythrocyte S-adenosylmethionine (SAM), S-adenosylhomocysteine (SAH) concentrations, SAM/SAH ratio, CpG methylation levels across the promoter region of the extracellular superoxide dismutase (ec-SOD) gene, and ec-SOD activity in healthy men. Methods: Serum folate levels were measured in 111 subjects who were categorized in quintiles according to their folate status. Subjects located at the lowest, middle, and upper quintiles were selected for assessment of SAM and SAH by high-performance liquid chromatography, C677T Genotype of the 5,10-methylenetetrahydrofolate reductase (MTHFR) gene, ec-SOD methylation of CpG sites in lymphocytes genomic DNA by bisulfate treatment, and ec-SOD activity by a chemical assay. Results: Sixteen subjects were in the lowest serum folate quintile (<23.6 nmol/L), 17 in the middle (>34-<42 nmol/L), and 14 in the highest (>45nmol/L). SAM concentration was higher in the upper than in the middle and lowest quintiles (5.57 +/- 1.58, 2.52 +/- 0.97 2.29 +/- 1.2 mu mol/L: P < 0.0001). SAH concentration was higher in the upper compared with the lowest quintile (0.76 +/- 0.24 versus 0.52 +/- 0.23 mu mol/L, P < 0.001). There were no differences in the SAM/SAH ratio, ec-SOD activity, methylation status of CpG sites of the ec-SOD gene, and TMTHFR C677T genotype between groups. Conclusion: Serum folate concentrations in the highest quintile among healthy humans are associated with increased erythrocyte SAM and SAH concentrations, but not with SAM/SAH ratio or with methylation levels of CpG sites across the promoter region of the ec-SOD gene. Further research is required to determine if these findings are beneficial or harmful.en_US
Patrocinadordc.description.sponsorshipFONDECYT 1050380en_US
Lenguagedc.language.isoenen_US
Publisherdc.publisherELSEVIERen_US
Keywordsdc.subjectEXTRACELLULAR-SUPEROXIDE-DISMUTASEen_US
Títulodc.titleMethylation status in healthy subjects with normal and high serum folate concentrationen_US
Document typedc.typeArtículo de revistaen_US


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