| Author | dc.contributor.author | Santibáñez, Juan Francisco | |
| Author | dc.contributor.author | Kocic, Jelena | es_CL |
| Author | dc.contributor.author | Fabra, Angels | es_CL |
| Author | dc.contributor.author | Cano, Amparo | es_CL |
| Author | dc.contributor.author | Quintanilla, Miguel | es_CL |
| Admission date | dc.date.accessioned | 2010-07-01T19:58:55Z | |
| Available date | dc.date.available | 2010-07-01T19:58:55Z | |
| Publication date | dc.date.issued | 2010 | |
| Cita de ítem | dc.identifier.citation | FEBS Letters 584 (2010) 2305–2310 | en_US |
| Identifier | dc.identifier.other | doi:10.1016/j.febslet.2010.03.042 | |
| Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/123968 | |
| Abstract | dc.description.abstract | Transforming growth factor-b1 (TGF-b1) activates Rac1 GTPase in mouse transformed keratinocytes.
Expression of a constitutively active Q61LRac1 mutant induced an epithelial to mesenchymal transition
(EMT) linked to stimulation of cell migration and invasion. On the contrary, expression of a
dominant-negative N17TRac1 abolished TGF-b1-induced cell scattering, migration and invasion.
Moreover, Q61LRac1 enhanced metalloproteinase-9 (MMP9) production to levels comparable to
those induced by TGF-b1, while N17TRac1 was inhibitory. TGF-b1-mediated EMT involves the expression
of the E-cadherin repressor Snail1, regulated by the Rac1 and mitogen-activated protein kinase
(MAPK) pathways. Furthermore, MMP9 production was MAPK-dependent, as the MEK inhibitor
PD98059 decreased TGF-b1-induced MMP9 expression and secretion in Q61LRac1 expressing cells.
We propose that regulation of TGF-b1-mediated plasticity of transformed keratinocytes requires
the cooperation between the Rac1 and MAPK signalling pathways. | en_US |
| Patrocinador | dc.description.sponsorship | The financial support of the Fund for Science and Technology of
Chile (grants FONDECYT #1050476 and # 3000045 to J.F.S.), the
University of Chile (DID # I003-99/2 to J.F.S.), the Ministry of Science
and Technological Development, Republic of Serbia (Grant
#145048) and the Spanish Ministry of Science and Innovation
(SAF2007-63821 to M.Q.) is gratefully acknowledged. | en_US |
| Lenguage | dc.language.iso | en | en_US |
| Publisher | dc.publisher | ELSEVIER | en_US |
| Keywords | dc.subject | Keratinocyte | en_US |
| Título | dc.title | Rac1 modulates TGF-b1-mediated epithelial cell plasticity and MMP9 production in transformed keratinocytes | en_US |
| Document type | dc.type | Artículo de revista | |
