Soluble factors derived from tumor mammary cell lines induce a stromal mammary adipose reversion in human and mice adipose cells. Possible role of TGF-b1 and TNF-a
Author | dc.contributor.author | Guerrero, Javier | |
Author | dc.contributor.author | Tobar, Nicolás | es_CL |
Author | dc.contributor.author | Cáceres, Mónica | es_CL |
Author | dc.contributor.author | Espinoza, Lorena | es_CL |
Author | dc.contributor.author | Escobar, Paula | es_CL |
Author | dc.contributor.author | Dotor, Javier | es_CL |
Author | dc.contributor.author | Smith, Patricio C. | es_CL |
Author | dc.contributor.author | Martínez, Jorge | es_CL |
Admission date | dc.date.accessioned | 2010-07-14T19:47:13Z | |
Available date | dc.date.available | 2010-07-14T19:47:13Z | |
Publication date | dc.date.issued | 2010 | |
Cita de ítem | dc.identifier.citation | Breast Cancer Res Treat (2010) 119:497–508 | en_US |
Identifier | dc.identifier.other | DOI 10.1007/s10549-009-0491-1 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/123972 | |
Abstract | dc.description.abstract | In carcinomas such as those of breast, pancreas, stomach, and colon, cancer cells support the expansion of molecular and cellular stroma in a phenomenon termed desmoplasia, which is characterized by a strong fibrotic response. In the case of breast tissue, in which stroma is mainly a fatty tissue, this response presumably occurs at the expense of the adipose cells, the most abundant stromal phenotype, generating a tumoral fibrous structure rich in fibroblast-like cells. In this study, we aimed to determine the cellular mechanisms by which factors present in the media conditioned by MDA-MB-231 and MCF-7 human breast cancer cell lines induce a reversion of adipose cells to a fibroblastic phenotype. We demonstrated that soluble factors generated by these cell lines stimulated the reversion of mammary adipose phenotype evaluated as intracellular lipid content and expression of C/EBPa and PPARc. We also demonstrated that exogenous TGF-b1 and TNF-a exerts a similar function. The participation of both growth factors, components of media conditioned by tumoral mammary cells, on the expression and nuclear translocation of C/EBPa and PPARc was tested in 3T3-L1 cells by interfering with the inhibitory effects of media with agents that block the TGF-b1 and TNF-a activity. These results allow us to postulate that TGF-b1 and TNF-a present in this media are in part responsible for this phenotypic reversion. | en_US |
Patrocinador | dc.description.sponsorship | This work was supported by the grant (1080196 to JM) from the Fondo Nacional de Ciencia y Tecnologı´a (FONDECYT) of Chile. | en_US |
Lenguage | dc.language.iso | en | en_US |
Publisher | dc.publisher | Springer | en_US |
Keywords | dc.subject | Desmoplasia | en_US |
Título | dc.title | Soluble factors derived from tumor mammary cell lines induce a stromal mammary adipose reversion in human and mice adipose cells. Possible role of TGF-b1 and TNF-a | en_US |
Document type | dc.type | Artículo de revista |
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