|Espejo Torres, Romilio
|Cita de ítem
|Journal of Fish Diseases 2012, 35, 193–201
|Flavobacterium psychrophilum causes rainbow trout
fry syndrome (RTFS) and cold water disease
(CWD) in salmonid aquaculture. We report characterization
of F. psychrophilum strains and their
bacteriophages isolated in Chilean salmonid aquaculture.
Results suggest that under laboratory conditions
phages can decrease mortality of salmonids
from infection by their F. psychrophilum host strain.
Twelve F. psychrophilum isolates were characterized,
with DNA restriction patterns showing low diversity
between strains despite their being obtained
from different salmonid production sites and from
different tissues. We isolated 15 bacteriophages able
to infect some of the F. psychrophilum isolates and
characterized six of them in detail. DNA genome
sizes were close to 50 Kbp and corresponded to the
Siphoviridae and Podoviridae families. One isolate,
6H, probably contains lipids as an essential virion
component, based on its chloroform sensitivity and
low buoyant density in CsCl. Each phage isolate
rarely infected F. psychrophilum strains other than
the strain used for its enrichment and isolation.
Some bacteriophages could decrease mortality from
intraperitoneal injection of its host strain when
added together with the bacteria in a ratio of 10
plaque-forming units per colony-forming unit.
While we recognize the artificial laboratory conditions
used for these protection assays, this work is
the first to demonstrate that phages might be able
protect salmonids from RTFS or CWD.
|This work was partially
supported by Grant INNOVA 07CN13PPT-09 of
CORFO, Chile, and by grants from the Directorate
for Food, Fisheries and Agri Business and the
Danish Natural Sciences Research Council to M.M.
|bacterial cold water disease
|Diversity of Flavobacterium psychrophilum and the potential use of its phages for protection against bacterial cold water disease in salmonids
|Artículo de revista