Proliferation and Differentiation of Human Adipocyte Precursor Cells: Differences Between the Preperitoneal and Subcutaneous Compartments
Author
dc.contributor.author
Fernández Gálvez, Mireya
es_CL
Author
dc.contributor.author
Acuña, M. J.
es_CL
Author
dc.contributor.author
Reyes, M.
es_CL
Author
dc.contributor.author
Olivares, D.
es_CL
Author
dc.contributor.author
Hirsch Birn, Sandra
es_CL
Author
dc.contributor.author
Bunout Barnett, Daniel
es_CL
Author
dc.contributor.author
Maza Cave, María Pía de la
Admission date
dc.date.accessioned
2014-01-02T17:56:37Z
Available date
dc.date.available
2014-01-02T17:56:37Z
Publication date
dc.date.issued
2010
Cita de ítem
dc.identifier.citation
Journal of Cellular Biochemistry 111:659–664 (2010)
en_US
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/124024
General note
dc.description
Acceso restringido a texto completo
en_US
Abstract
dc.description.abstract
Human adipocyte precursor cells (APC) have been characterized in their proliferation and differentiation potential from subcutaneous,
omental, and mesenteric depots, mostly from morbidly obese patients. Cells from the preperitoneal adipose compartment have not been
characterized yet, least of all when obtained from normal weight subjects. The aim was to compare proliferation and differentiation of
subcutaneous (SC) and preperitoneal (PP) APC derived from adipose tissue in healthy subjects with different body mass. SC and PP adipose
tissue was obtained during surgery of inguinal hernias in five healthy non-obese subjects and three obese otherwise healthy men. APC,
obtained by collagenase digestion, were cultured. Proliferation was assayed by cell counting and differentiation by oil red O staining and flow
cytometry using Nile Red staining. Proliferation of SC was higher than PP APC. Such differences between both compartments were even
higher in APC obtained from obese patients. Conversely PP APC differentiated earlier in vitro compared with SC cells. These results agree with
published data on fat cell proliferation. However regarding differentiation, our data show that APC from deeper depots (in this case PP)
differentiate earlier than subcutaneous APC. This is different to previous studies performed in mesenteric or omental adipose tissue