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Authordc.contributor.authorFernández Gálvez, Mireya es_CL
Authordc.contributor.authorAcuña, M. J. es_CL
Authordc.contributor.authorReyes, M. es_CL
Authordc.contributor.authorOlivares, D. es_CL
Authordc.contributor.authorHirsch Birn, Sandra es_CL
Authordc.contributor.authorBunout Barnett, Daniel es_CL
Authordc.contributor.authorMaza Cave, María Pía de la 
Cita de ítemdc.identifier.citationJournal of Cellular Biochemistry 111:659–664 (2010)en_US
General notedc.descriptionAcceso restringido a texto completoen_US
Abstractdc.description.abstractHuman adipocyte precursor cells (APC) have been characterized in their proliferation and differentiation potential from subcutaneous, omental, and mesenteric depots, mostly from morbidly obese patients. Cells from the preperitoneal adipose compartment have not been characterized yet, least of all when obtained from normal weight subjects. The aim was to compare proliferation and differentiation of subcutaneous (SC) and preperitoneal (PP) APC derived from adipose tissue in healthy subjects with different body mass. SC and PP adipose tissue was obtained during surgery of inguinal hernias in five healthy non-obese subjects and three obese otherwise healthy men. APC, obtained by collagenase digestion, were cultured. Proliferation was assayed by cell counting and differentiation by oil red O staining and flow cytometry using Nile Red staining. Proliferation of SC was higher than PP APC. Such differences between both compartments were even higher in APC obtained from obese patients. Conversely PP APC differentiated earlier in vitro compared with SC cells. These results agree with published data on fat cell proliferation. However regarding differentiation, our data show that APC from deeper depots (in this case PP) differentiate earlier than subcutaneous APC. This is different to previous studies performed in mesenteric or omental adipose tissueen_US
Patrocinadordc.description.sponsorshipFONDECyT; Grant number: 1030597.en_US
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.uri*
Títulodc.titleProliferation and Differentiation of Human Adipocyte Precursor Cells: Differences Between the Preperitoneal and Subcutaneous Compartmentsen_US
Document typedc.typeArtículo de revistaen_US

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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile