Soluble factors derived from tumor mammary cell lines induce a stromal mammary adipose reversion in human and mice adipose cells. Possible role of TGF-b1 and TNF-a
Author
dc.contributor.author
Guerrero, Javier
Author
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Tobar, Nicolás
es_CL
Author
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Cáceres, Mónica
es_CL
Author
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Espinoza, Lorena
es_CL
Author
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Escobar Oliva, Paula
es_CL
Author
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Dotor, Javier
es_CL
Author
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Smith, Patricio C.
es_CL
Author
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Martínez Winkler, Jorge
es_CL
Admission date
dc.date.accessioned
2014-01-08T14:37:56Z
Available date
dc.date.available
2014-01-08T14:37:56Z
Publication date
dc.date.issued
2011
Cita de ítem
dc.identifier.citation
Breast Cancer Res Treat (2010) 119:497–508. DOI 10.1007/s10549-009-0491-1
en_US
Identifier
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1573-7217
Identifier
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https://repositorio.uchile.cl/handle/2250/124046
General note
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Artículo de publicación ISI
en_US
Abstract
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In carcinomas such as those of breast, pancreas,
stomach, and colon, cancer cells support the expansion of
molecular and cellular stroma in a phenomenon termed
desmoplasia, which is characterized by a strong fibrotic
response. In the case of breast tissue, in which stroma is
mainly a fatty tissue, this response presumably occurs at
the expense of the adipose cells, the most abundant stromal
phenotype, generating a tumoral fibrous structure rich in
fibroblast-like cells. In this study, we aimed to determine
the cellular mechanisms by which factors present in the
media conditioned by MDA-MB-231 and MCF-7 human
breast cancer cell lines induce a reversion of adipose cells
to a fibroblastic phenotype. We demonstrated that soluble
factors generated by these cell lines stimulated the reversion
of mammary adipose phenotype evaluated as intracellular
lipid content and expression of C/EBPa and
PPARc. We also demonstrated that exogenous TGF-b1 and
TNF-a exerts a similar function. The participation of both
growth factors, components of media conditioned by
tumoral mammary cells, on the expression and nuclear
translocation of C/EBPa and PPARc was tested in 3T3-L1
cells by interfering with the inhibitory effects of media
with agents that block the TGF-b1 and TNF-a activity.
These results allow us to postulate that TGF-b1 and TNF-a
present in this media are in part responsible for this phenotypic
reversion.
en_US
Patrocinador
dc.description.sponsorship
This work was supported by the grant (1080196
to JM) from the Fondo Nacional de Ciencia y Tecnologı´a (FONDECYT)
of Chile.
Soluble factors derived from tumor mammary cell lines induce a stromal mammary adipose reversion in human and mice adipose cells. Possible role of TGF-b1 and TNF-a