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Authordc.contributor.authorRodríguez, J. Pablo 
Authordc.contributor.authorMontecinos, Luis es_CL
Authordc.contributor.authorRíos, Susana es_CL
Authordc.contributor.authorReyes, Patricio es_CL
Authordc.contributor.authorMartínez, Jorge es_CL
Cita de ítemdc.identifier.citationJournal of Cellular Biochemistry 79:557–565 (2000)en_US
General notedc.descriptionArtículo de publicación ISIen_US
Abstractdc.description.abstractMesenchymal stem cells (MSCs), precursor cells resident in the bone marrow, have the capacity to differentiate into bone, cartilage, fat, and connective tissue. We have recently reported that MSCs from "healthy" donors differ from cells obtained from osteoporotic postmenopausal women in their proliferation rate, mitogenic response to osteogenic growth factors, and potential to mineralize. The purpose of this study was to examine the factors that explain the differential capacity of MSCs derived from "healthy" control and osteoporotic postmenopausal women to support mineralization. In addition, we examined the factors that regulate the differentiation of osteoporotic cells into adipocytes. For this purpose, Lye isolated MSCs from bone marrow of donors and analyzed the synthesis and deposition of type I collagen, the main component of bone extracellular matrix, the time course of gelatinolytic activity expression, the deposition of transforming growth factor beta (TGF-beta), and the ability of cells to differentiate into adipocytes. Our results indicate that cells derived from osteoporotic donors synthesized 50% less type I collagen than normal cells and maintained higher levels of gelatinolytic activity under differentiation conditions (70% versus 15% after 14 days in culture). MSCs derived from osteoporotic women produced 60-65% less TGF-beta and expressed higher adipogenic capacity. We conclude that the capacity of MSCs derived from osteoporotic postmenopausal women to generate and maintain type I collagen-rich extracellular matrix is decreased, favoring their adipogenic differentiation. These observations may explain the decreased mineralization previously observed in these types of cells.en_US
Patrocinadordc.description.sponsorshipFondo Nacional de Desarrollo Cientitico y Tecnologies; Grant number: 8970028.en_US
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.uri*
Títulodc.titleMesenchymal Stem Cells from Osteoporotic Patients Produce a Type I Collagen-Deficient Extracellular Matrix Favoring Adipogenic Differentiationen_US
Document typedc.typeArtículo de revista

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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile