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Authordc.contributor.authorRojas Baechler, Cecilia 
Authordc.contributor.authorMartínez, Jessica I. es_CL
Authordc.contributor.authorFlores, Ingrid es_CL
Authordc.contributor.authorHoffman, Dennis R. es_CL
Authordc.contributor.authorUauy Dagach-Imbarack, Ricardo es_CL
Cita de ítemdc.identifier.citationInvest Ophthalmol Vis Sci. 2003;44:3170–3177en_US
Identifierdc.identifier.otherDOI: 10.1167/iovs.02-1138
General notedc.descriptionArtículo de publicación ISIen_US
Abstractdc.description.abstractPURPOSE. To explore the effect of docosahexaenoic acid (DHA) on gene expression during human fetal retinal maturation. METHODS. Human fetal retinal explants were cultured in serum-free Waymouth's medium supplemented with DRA or oleic acid (OA), using bovine serum albumin (BSA) as the vehicle., After 14 days in culture, fatty acid composition was assessed, and the abundance of 2400 cDNAs was examined with a human cDNA microarray system. RESULTS. Transcript abundance remained unchanged for 82% and 90% of genes in the explants with added DRA or OA, respectively. Decreased expression was detected in 4% and 9% of genes, in explants supplemented with DHA or OA, respectively, whereas, 14% of genes in explants exposed to DHA and only 0.4% of genes in explants treated with OA showed increased expression. Transcripts displaying changes in abundance in explants supplemented with DRA encode for proteins involved in diverse biological functions, including neurogenesis, neurotransmission, and refinement of connectivity. These gene expression changes were not observed in explants supplemented with OA. CONCLUSIONS. The effect of DRA deficiency on retinal function during human development can be partly explained by modifications in retinal gene expression by direct or indirect mechanisms.en_US
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.uri*
Keywordsdc.subjectVERTEBRATE RETINAen_US
Títulodc.titleGene Expression Analysis in Human Fetal Retinal Explants Treated with Docosahexaenoic Aciden_US
Document typedc.typeArtículo de revista

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Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile