Polyphenols Protect the Epithelial Barrier Function of Caco‑2 Cells Exposed to Indomethacin through the Modulation of Occludin and Zonula Occludens‑1 Expression
Author
dc.contributor.author
Carrasco Pozo, Catalina
Author
dc.contributor.author
Morales, Pamela
es_CL
Author
dc.contributor.author
Gotteland, Martín
es_CL
Admission date
dc.date.accessioned
2014-03-07T13:29:14Z
Available date
dc.date.available
2014-03-07T13:29:14Z
Publication date
dc.date.issued
2013
Cita de ítem
dc.identifier.citation
J. Agric. Food Chem. 2013, 61, 5291−5297
en_US
Identifier
dc.identifier.other
doi 10.1021/jf400150p
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/124104
General note
dc.description
Artículo de publicación ISI
en_US
Abstract
dc.description.abstract
The aim of this study was to determine the protective effect of quercetin, epigallocatechingallate, resveratrol, and
rutin against the disruption of epithelial integrity induced by indomethacin in Caco-2 cell monolayers. Indomethacin decreased
the transepithelial electrical resistance and increased the permeability of the monolayers to fluorescein−dextran. These alterations
were abolished by all the tested polyphenols but rutin, with quercetin being the most efficient. The protective effect of quercetin
was associated with its capacity to inhibit the redistribution of ZO-1 protein induced in the tight junction by indomethacin or
rotenone, a mitochondrial complex-I inhibitor, and to prevent the decrease of ZO-1 and occludin expression induced by
indomethacin. The fact that the antioxidant polyphenols assayed in this study differ in their protective capacity against the
epithelial damage induced by indomethacin suggests that this damage is due to the ability of this agent to induce not only
oxidative stress but also mitochondrial dysfunction.
Polyphenols Protect the Epithelial Barrier Function of Caco‑2 Cells Exposed to Indomethacin through the Modulation of Occludin and Zonula Occludens‑1 Expression