Assessing chaperone for Zn, Cu-superoxide dismutase as an indicator of copper deficiency in malnourished children
Author
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Arredondo Olguín, Miguel
Author
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Weisstaub Nuta, Sergio
es_CL
Author
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Medina, Marcos
es_CL
Author
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Suazo Romo, Miriam
es_CL
Author
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Guzmán, Miguel
es_CL
Author
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Araya, Magdalena
es_CL
Admission date
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2014-12-12T12:00:30Z
Available date
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2014-12-12T12:00:30Z
Publication date
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2014
Cita de ítem
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Journal of Trace Elements in Medicine and Biology 28 (2014) 23– 27
en_US
Identifier
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DOI: 10.1016/j.jtemb.2013.10.004
Identifier
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https://repositorio.uchile.cl/handle/2250/124113
General note
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Artículo de publicación ISI
en_US
Abstract
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It is not clear how frequent is copper deficiency in humans. Current copper markers are not sensitive
enough to detect early copper deficiency and new markers are needed. CCS is a candidate to become a
copper biomarker.
Objective: Measuring CCS mRNA relative expression in malnourished children and compare results (a)
with those of the same children after nutritional recovery and (b) with well-nourished children.
Method: On admission to the protocol and after 15 day nutritional treatment, severely (G1 = 18) and moderately
(G2 = 10) malnourished children were compared with well-nourished healthy controls (G3 = 15),
measuring anthropometric indicators, blood biochemistry, Cu, Fe and Zn serum concentrations, ceruloplasmin,
C Reactive protein and mRNA abundance of CCS, SOD and MT2 in peripheral mononuclear
cells.
Result: In malnourished groups, mean serum copper concentration was below the cut-off on admission
to hospital and increased after 15 days (t-test, p < 0.01). On admission to protocol, CCS mRNA abundance
in G1 and G2 was higher than in G3 (one way ANOVA, p < 0.001). After 15 days, CCS expression decreased
as expected (t-test, p < 0.001). Initial SOD mRNA relative abundance was higher in study groups than
controls and also between G1 and G2 (One way ANOVA, both p < 0.01); after 15 days, G1 and G2 were not
different (t-test, NS). MT2A abundance of transcripts did not follow a clear change pattern.
Conclusion: CCS mRNA abundance responded as expected, being higher in malnourished children than
in controls; nutritional recovery in these latter resulted in decreasing expression of the chaperone,
supporting the hypothesis that CCS may be a copper biomarker.
en_US
Patrocinador
dc.description.sponsorship
This project was supported by Fondecyt grant 1110099.