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Rhythmic expression of functional MT1 melatonin receptors in the rat adrenal gland

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2008-03
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Richter, Hans G.
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Rhythmic expression of functional MT1 melatonin receptors in the rat adrenal gland
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  • Richter, Hans G.;
  • Torres Farfán, Claudia;
  • García Sesnich, Jocelyn;
  • Abarzúa Catalán, Lorena;
  • Henríquez Luna, Mauricio;
  • Alvarez Felmer, Mauro;
  • Gaete, Fernando;
  • Rehren, Germán E.;
  • Serón Ferré, María;
Abstract
We previously demonstrated that melatonin is involved in the regulation of adrenal glucocorticoid production in diurnal primates through activation of MT1 membrane-bound melatonin receptors. However, whether melatonin has a similar role in nocturnal rodents remains unclear. Using an integrative approach, here we show that the adult rat adrenal gland expresses a functional MT1 melatonin receptor in a rhythmic fashion. We found that: 1) expression of the cognate mRNA encoding for the MT1 membrane-bound melatonin receptor, displaying higher levels in the day/night transition (1800–2200 h); 2) expression of the predicted 37-kDa MT1 polypeptide in immunoblots from adrenals collected at 2200 h but not 1000 h; 3) no expression of the MT2 melatonin receptor mRNA and protein; 4) specific high-affinity 2-[125I]iodomelatonin binding in membrane fractions and frozen sections from adrenals collected at 2200 h but not 0800 h (dissociation constant 14.22 1.23 pM; maximal binding capacity 0.88 0.02 fmol/mg protein); and 5) in vitro clock time-dependent inhibition of ACTHstimulated corticosterone production by 1–100 nM melatonin, which was reversed by 1 M luzindole (a melatonin membrane receptor antagonist). Our findings indicate not only expression but also high amplitude diurnal variation of functional MT1 melatonin receptors in the rat adrenal gland. It is conceivable that plasma melatonin may play a role to fine-tune corticosterone production in nocturnal rodents, probably contributing to the down slope of the corticosterone rhythm.
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This work was supported by Grants 1050389 from Fondo Nacional de Desarrollo Cientifico y Tecnologico and S-2004-33 from Direccion de Investigacion y Desarrollo de la Universidad Austral de Chile (DIDUACh), Chile (to H.G.R.)
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URI: https://repositorio.uchile.cl/handle/2250/128399
ISSN: 0013-7227
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ENDOCRINOLOGY, Volume: 149, Issue: 3, Pages: 995-1003, 2008
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