Morfometría ovárica y expresión del ARN mensajero de hormona antimülleriana (AMH), receptor de FSH (FSHR) y factor nuclear kappa B (NFkB) en folículos en crecimiento de borregas expuestas prenatalmente a testosterona
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Rojas García, Pedro
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Morfometría ovárica y expresión del ARN mensajero de hormona antimülleriana (AMH), receptor de FSH (FSHR) y factor nuclear kappa B (NFkB) en folículos en crecimiento de borregas expuestas prenatalmente a testosterona
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Abstract
Antenatal exposure to testosterone (T) has a series of consequences on postnatal reproductive parameters in females of several animal species. In
sheep born under this condition the ovaries are characterized by an increased number of growing follicles. If such disruptions are manifested early in
life or involve changes in follicular recruitment and ovary paracrine environment, it remains unclear. This study addressed the impact of prenatal T on
ovarian morphometry and the mRNA expression of ovarian anti-müllerian hormone (AMH), FSH receptor (FSHR) and nuclear factor kappa B (NFkB,
a transcription factor for AMH), factors that are involved in the growth and selection of the dominant follicle. At 4 weeks of age, female lambs born to
dams treated with 30 mg of T propionate twice weekly from day 30 to day 90, followed by 40 mg of T propionate from day 90 to day 120 of pregnancy
(T females), showed similar number of primordial, primary, secondary and antral follicles than control females (C females) born to dams treated with the
vehicle. In the pool of secondary follicles (< 0.5 mm) the expression of the FSHR was slightly higher in the T females, while AMH and NFkB expression
were lower than C females. Instead, the pool of small antral follicles (between 0.5 and 1 mm) showed a higher expression of FSHR and a slight increase
in AMH in T females, while NFkB expression remained lower than C females. These findings provide evidence that the prenatal exposure to T impacts
the expression of key local factors governing the folliculogenesis before histological alterations occur.
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Financiado por proyecto Fondecyt 1090031
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URI: https://repositorio.uchile.cl/handle/2250/129590
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Arch Med Vet 46, 13-21 (2014)
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