Genetically engineered Thompson Seedless grapevine plants designed for fungal tolerance: selection and characterization of the best performing individuals in a field trial
Author
dc.contributor.author
Rubio Astudillo, Julia
Author
dc.contributor.author
Montes, Christian
Author
dc.contributor.author
Castro, Álvaro
Author
dc.contributor.author
Álvarez, Catalina
Author
dc.contributor.author
Olmedo, Blanca
Author
dc.contributor.author
Muñoz, Marisol
Author
dc.contributor.author
Tapia, Eduardo
Author
dc.contributor.author
Reyes, Fernando
Author
dc.contributor.author
Ortega, Marcelo
Author
dc.contributor.author
Sánchez, Evelyn
Author
dc.contributor.author
Miccono, María
Author
dc.contributor.author
Dalla Costa, Lorenza
Author
dc.contributor.author
Martinelli, Lucia
Author
dc.contributor.author
Malnoy, Mickael
Author
dc.contributor.author
Prieto, Humberto
Admission date
dc.date.accessioned
2015-08-20T02:50:41Z
Available date
dc.date.available
2015-08-20T02:50:41Z
Publication date
dc.date.issued
2015
Cita de ítem
dc.identifier.citation
Transgenic Res (2015) 24:43–60
en_US
Identifier
dc.identifier.other
DOI: 10.1007/s11248-014-9811-2
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/132949
General note
dc.description
Artículo de publicación ISI
en_US
Abstract
dc.description.abstract
The fungi Botrytis cinerea and Erysiphe
necator are responsible for gray mold and powdery
mildew diseases, respectively, which are among the
most devastating diseases of grapes. Two endochitinase
(ech42 and ech33) genes and one N-acetyl-b-Dhexosaminidase
(nag70) gene from biocontrol agents
related to Trichoderma spp. were used to develop a set
of 103 genetically modified (GM) ‘Thompson Seedless’
lines (568 plants) that were established in open
field in 2004 and evaluated for fungal tolerance
starting in 2006. Statistical analyses were carried out
considering transgene, explant origin, and plant
response to both fungi in the field and in detached
leaf assays. The results allowed for the selection of the
19 consistently most tolerant lines through two
consecutive years (2007–2008 and 2008–2009 seasons).
Plants from these lines were grafted onto the
rootstock Harmony and established in the field in 2009
for further characterization. Transgene status was
shown in most of these lines by Southern blot, realtime
PCR, ELISA, and immunostrips; the most
tolerant candidates expressed the ech42–nag70 double
gene construct and the ech33 gene from a local
Hypocrea virens isolate. B. cinerea growth assays in
Petri dishes supplemented with berry juices extracted
from the most tolerant individuals of the selected
population was inhibited. These results demonstrate
that improved fungal tolerance can be attributed to
transgene expression and support the iterative molecular
and physiological phenotyping in order to define
selected individuals from a population of GM
grapevines.
Genetically engineered Thompson Seedless grapevine plants designed for fungal tolerance: selection and characterization of the best performing individuals in a field trial