Inactivated E. coli transformed with plasmids that produce dsRNA against infectious salmon anemia virus hemagglutinin show antiviral activity when added to infected ASK cells
| Author | dc.contributor.author | Katherin, García | |
| Author | dc.contributor.author | Ramírez Araya, Sebastián | |
| Author | dc.contributor.author | Díaz, Álvaro | |
| Author | dc.contributor.author | Reyes Cerpa, Sebastián | |
| Author | dc.contributor.author | Espejo Torres, Romilio | |
| Author | dc.contributor.author | Higuera, Gastón | |
| Author | dc.contributor.author | Higuera, Gastón | |
| Author | dc.contributor.author | Romero Ormazábal, Jaime | |
| Admission date | dc.date.accessioned | 2015-08-25T21:21:17Z | |
| Available date | dc.date.available | 2015-08-25T21:21:17Z | |
| Publication date | dc.date.issued | 2015 | |
| Cita de ítem | dc.identifier.citation | Volumen: 6 Número de artículo: 300 | en_US |
| Identifier | dc.identifier.issn | 1664-302X | |
| Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/133160 | |
| General note | dc.description | Artículo de publicación ISI. | en_US |
| Abstract | dc.description.abstract | Infectioussalmonanemiavirus(ISAV)hascausedgreatlossestotheChileansalmonindustry,andthesuccessofpreventionandtreatmentstrategiesisuncertain.TheuseofRNAinterference(RNAi)isapromisingapproachbecauseduringthereplicationcycle,theISAVgenomemustbetranscribedtomRNAinthecytoplasm.WeexploredthecapacityofE.colitransformedwithplasmidsthatproducedouble-strandedRNA(dsRNA)toinduceantiviralactivitywhenaddedtoinfectedASKcells.Wetransformedthenon-pathogenicEscherichiacoliHT115(DE3)withplasmidsthatexpressedhighlyconservedregionsoftheISAVgenesencodingthenucleoprotein(NP),fusion(F),hemagglutinin(HE),andmatrix(M)proteinsasdsRNA,whichistheprecursoroftheRNAimechanism.TheinactivatedtransformedbacteriacarryingdsRNAweretestedfortheircapacitytosilencethetargetISAVgenes,andthedsRNAthatwereabletoinhibitgeneexpressionweresubsequentlytestedfortheirabilitytoattenuatethecytopathiceffect(CPE)andreducetheviralload.Ofthefourtargetgenestested,inactivatedE.colitransformedwithplasmidsproducingdsRNAtargetingHEshowedantiviralactivitywhenaddedtoinfectedASKcells. | en_US |
| Patrocinador | dc.description.sponsorship | FONDEF, FONDECYT Postdoctorado | en_US |
| Lenguage | dc.language.iso | en | en_US |
| Publisher | dc.publisher | FRONTIERS RESEARCH FOUNDATION | en_US |
| Type of license | dc.rights | Atribución-NoComercial-SinDerivadas 3.0 Chile | * |
| Link to License | dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | * |
| Keywords | dc.subject | aquaculture | en_US |
| Keywords | dc.subject | antiviral | en_US |
| Keywords | dc.subject | bacterial delivery | en_US |
| Keywords | dc.subject | infectious salmon anemia virus | en_US |
| Keywords | dc.subject | double-stranded RNA | en_US |
| Título | dc.title | Inactivated E. coli transformed with plasmids that produce dsRNA against infectious salmon anemia virus hemagglutinin show antiviral activity when added to infected ASK cells | en_US |
| Document type | dc.type | Artículo de revista |
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Except where otherwise noted, this item's license is described as Atribución-NoComercial-SinDerivadas 3.0 Chile