Antioxidant Capacities and Analysis of Phenolic Compounds in Three Endemic Nolana Species by HPLC-PDA-ESI-MS
Author
dc.contributor.author
Areche Medina, Carlos
Admission date
dc.date.accessioned
2015-10-08T19:22:50Z
Available date
dc.date.available
2015-10-08T19:22:50Z
Publication date
dc.date.issued
2015
Cita de ítem
dc.identifier.citation
Molecules 2015, 20, 11490-11507
en_US
Identifier
dc.identifier.other
DOI: 10.3390/molecules200611490
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/134287
General note
dc.description
Artículo de publicación ISI
en_US
Abstract
dc.description.abstract
The antioxidant features, polyphenolic composition and chromatographic fingerprints of the aerial parts from three Chilean endemic plants from the Paposo Valley located on the cost of the Atacama Desert were investigated for the first time using high pressure liquid chromatography coupled with photodiode array detector and electrospray ionization mass analysis (HPLC-PDA-ESI-MS) and spectroscopic methods. The phenolic fingerprints obtained for the plants were compared and correlated with the antioxidant capacities measured by the bleaching of the DPPH radical, the ferric reducing antioxidant power (FRAP) and quantification of the total content of phenolics and flavonoids measured by spectroscopic methods. Thirty phenolics were identified for the first time for these species, mostly phenolic acids, flavanones, flavonols and some of their glycoside derivatives, together with three saturated fatty acids (stearic, palmitic and arachidic acids). Nolana ramosissima showed the highest antioxidant activity (26.35 +/- 1.02 g/mL, 116.07 +/- 3.42 M Trolox equivalents/g dry weight and 81.23% +/- 3.77% of inhibition in the DPPH, FRAP and scavenging activity (SA) assays, respectively), followed by N. aplocaryoides (85.19 +/- 1.64 g/mL, 65.87 +/- 2.33 M TE/g DW and 53.27% +/- 3.07%) and N. leptophylla (124.71 +/- 3.01, 44.23 +/- 5.18 M TE/g DW and 38.63% +/- 1.85%).
en_US
Patrocinador
dc.description.sponsorship
National Fund of Scientific and Technological Development of Chile (Fondecyt)
1140178
FONDEQUIP
masas-TOF-EQM14002