Mechanism of potassium ion uptake by the Na+/K+-ATPase
Author
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Castillo, Juan P.
Author
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Rui, Huan
Author
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Basilio Seyler, Daniel
Author
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Das, Avisek
Author
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Roux, Benoit
Author
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Latorre, Ramón
Author
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Bezanilla, Francisco
Author
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Holmgren, Miguel
Admission date
dc.date.accessioned
2015-10-16T19:36:35Z
Available date
dc.date.available
2015-10-16T19:36:35Z
Publication date
dc.date.issued
2015
Cita de ítem
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Nature Communications 6:7622 2015
en_US
Identifier
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DOI: 10.1038/ncomms8622
Identifier
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https://repositorio.uchile.cl/handle/2250/134452
General note
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Artículo de publicación ISI
en_US
Abstract
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The Na+/ K+-ATPase restores sodium (Na+) and potassium (K+) electrochemical gradients dissipated by action potentials and ion-coupled transport processes. As ions are transported, they become transiently trapped between intracellular and extracellular gates. Once the external gate opens, three Na+ ions are released, followed by the binding and occlusion of two K+ ions. While the mechanisms of Na+ release have been well characterized by the study of transient Na+ currents, smaller and faster transient currents mediated by external K+ have been more difficult to study. Here we show that external K+ ions travelling to their binding sites sense only a small fraction of the electric field as they rapidly and simultaneously become occluded. Consistent with these results, molecular dynamics simulations of a pump model show a wide water-filled access channel connecting the binding site to the external solution. These results suggest a mechanism of K+ gating different from that of Na+ occlusion.
en_US
Patrocinador
dc.description.sponsorship
Fogarty International Research Collaboration Award
RO3 TW008351
NIH
R01-GM062342
R01-GM030376
U54-GM087519
FONDECYT
1110430
1150273
Millennium Scientific Initiative of the Chilean Ministry of Economy, Development and Tourism
NIH (NINDS)
National Institutes of Health
P41GM103712-S1
Pittsburgh Supercomputing Center (PSC)