High-throughput transcriptome analysis of ISAV-infected Atlantic salmon Salmo salar unravels divergent immune responses associated to head-kidney, liver and gills tissues
Author
dc.contributor.author
Valenzuela Miranda, Diego
Author
dc.contributor.author
Boltaña, Sebastián
Author
dc.contributor.author
Cabrejos, María Eugenia
Author
dc.contributor.author
Yáñez López, José
Author
dc.contributor.author
Gallardo Escárate, Cristian
Admission date
dc.date.accessioned
2015-10-27T19:07:43Z
Available date
dc.date.available
2015-10-27T19:07:43Z
Publication date
dc.date.issued
2015
Cita de ítem
dc.identifier.citation
Fish & Shellfish Immunology 45 (2015) 367-377
en_US
Identifier
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DOI: 10.1016/j.fsi.2015.04.003
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/134705
General note
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Artículo de publicación ISI
en_US
Abstract
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Infectious salmon anaemia virus (ISAV) is an orthomyxovirus causing high mortality in farmed Atlantic
salmon (Salmo salar). The collective data from the Atlantic salmoneISAV interactions, performed
“in vitro” using various salmon cell lines and “in vivo” fish infected with different ISAV isolates, have
shown a strong regulation of immune related transcripts during the infection. Despite this strong
defence response, the majority of fish succumb to infections with ISAV. The deficient protection of the
host against ISAV is in part due to virulence factors of the virus, which allow evade the host-defence
machinery. As such, the viral replication is uninhibited and viral loads quickly spread to several tissues
causing massive cellular damage before the host can develop an effective cell-mediated and humoral
outcome. To interrogate the correlation of the viral replication with the host defence response, we
used fish that have been infected by cohabitation with ISAV-injected salmons. Whole gene expression
patterns were measured with RNA-seq using RNA extracted from Head-kidney, Liver and Gills. The results
show divergent mRNA abundance of functional modules related to interferon pathway, adaptive/
innate immune response and cellular proliferation/differentiation. Furthermore, gene regulation in
distinct tissues during the infection process was independently controlled within the each tissue and the
observed mRNA expression suggests high modulation of the ISAV-segment transcription. Importantly
this is the first time that strong correlations between functional modules containing significant immune
process with proteineprotein affinities and viral-segment transcription have been made between
different tissues of ISAV-infected fish.
en_US
Patrocinador
dc.description.sponsorship
FONDAP project
1510027
CORFO INNOVA grant
09-MCSS6682