Extraction and Determination of Phenolic Compounds in the Berries of Sorbus americana Marsh and Lonicera oblongifolia (Goldie) Hook

Abstract

Phenolic compound content in Sorbus americana Marsh and Lonicera oblongifolia (Goldie) Hook berries was determined for the first time. An improved solid-liquid microextraction (SLME) method combining with highperformance liquid chromatography (HPLC)-diode array detector (DAD)-mass spectrometry (MS) has been developed to determine the phenolic compounds present in these berries, reducing the amount of sample, reagents, and time consumed. The major phenolic compound identified and quantified was 3-O-caffeoylquinic acid (3- CQA) in both berries. To a lesser extent, 5-Ocaffeoylquinic acid (5-CQA) and quercetin-3-O-glucoside (QG) were also determined. The existence of these phenolic compounds and the great abundance of these fruits in the northeast of North America make S. americana Marsh and L. oblongifolia Hook berries a new and excellent source of natural phenolic compounds (antioxidants), which can be very useful in biotechnological exploitation.