Exoenzymes and metabolites related to the nematicidal effect of rhizobacteria on Xiphinema index Thorne & Allen
Author
dc.contributor.author
Castañeda Álvarez, C.
Author
dc.contributor.author
Prodan, Simona
Author
dc.contributor.author
Rosales, I. M.
Author
dc.contributor.author
Aballay Espinoza, Erwin
Admission date
dc.date.accessioned
2016-06-13T20:13:05Z
Available date
dc.date.available
2016-06-13T20:13:05Z
Publication date
dc.date.issued
2016
Cita de ítem
dc.identifier.citation
Journal of Applied Microbiology Volumen: 120 Número: 2 Páginas: 413-424 Feb 2016
en_US
Identifier
dc.identifier.other
DOI: 10.1111/jam.12987
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/138771
General note
dc.description
Artículo de publicación ISI
en_US
General note
dc.description
Sin acceso a texto completo
Abstract
dc.description.abstract
AimsTo identify enzymes and metabolites in the rhizobacteria filtrates that have a nematicidal effect
on Xiphinema index and perform molecular characterization of the strains evaluated.
Methods and ResultsA series of four bacteria selected for their nematicidal potential were
considered for invitro, biochemical and molecular studies. The direct effect of the bacterial filtrates
was evaluated invitro on X.index juveniles and adults. Hydrogen sulphide and hydrogen cyanide
liberation and protease, chitinase, collagenase and lipase activity were verified in the strains. Up to
five housekeeping genes and one ITS 16S-23S rRNA were analysed. All bacterial filtrates
presented 54-100% mortality when evaluated during up to 72h of nematode exposure. Strains
presented protease activity; two of them (strains FB833T and FR203A) showed reliable collagenase
and chitinase activities, respectively, and three of them showed strong lipolytic activity (FB833T,
FR203A and FS213P). Strain Bacillus megaterium FB133M had no lipase activity and presented
the lowest nematicidal effect. Bacillus amyloliquefaciens FR203A had the largest lethal effect.
ConclusionThe rhizobacteria strains evaluated in this study possess nematicidal compounds, which
may offer an interesting alternative for X.index control.
Significance and Impact of the StudyThis is the first report of exoenzymes and metabolites
associated with nematicidal effect of rhizobacteria on X.index, which can be a possible alternative
for control of this plant-parasitic nematode.