Platelet-Poor and Platelet-Rich Plasma Stimulate Bone Lineage Differentiation in Periodontal Ligament Stem Cells
Author
dc.contributor.author
Martínez, Constanza E.
Author
dc.contributor.author
González, Sergio A.
Author
dc.contributor.author
Palma Alvarado, Verónica
Author
dc.contributor.author
Smith, Patricio C.
Admission date
dc.date.accessioned
2016-07-04T16:39:50Z
Available date
dc.date.available
2016-07-04T16:39:50Z
Publication date
dc.date.issued
2016
Cita de ítem
dc.identifier.citation
Journal of Periodontology Volumen: 87 Número: 2 Páginas: E18-E26 Feb 2016
en_US
Identifier
dc.identifier.other
DOI: 10.1902/jop.2015.150360
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/139407
General note
dc.description
Artículo de publicación ISI
en_US
General note
dc.description
Sin acceso a texto completo
Abstract
dc.description.abstract
Background: Plasma-derived fractions have been used as an autologous source of growth factors;
however, limited knowledge concerning their biologic effects has hampered their clinical application.
In this study, the authors analyze the content and specific effect of both platelet-rich plasma (PRP)
and platelet-poor plasma (PPP) on osteoblastic differentiation using primary cultures of human
periodontal ligament stem cells (HPLSCs).
Methods: The authors evaluated the growth factor content of PRP and PPP using a proteome
profiler array and enzyme-linked immunosorbent assay. HPLSCs were characterized by flow
cytometry and differentiation assays. The effect of PRP and PPP on HPLSC bone differentiation
was analyzed by quantifying calcium deposition after 14 and 21 days of treatment.
Results: Albeit at different concentrations, the two fractions had similar profiles of growth factors,
the most representative being platelet-derived growth factor (PDGF) isoforms (PDGF-AA, -BB, and
-AB), insulin-like growth factor binding protein (IGFBP)-2, and IGFBP-6. Both formulations exerted a
comparable stimulus on osteoblastic differentiation even at low doses (2.5%), increasing calcium
deposits in HPLSCs.
Conclusions: PRP and PPP showed a similar protein profile and exerted comparable effects on
bone differentiation. Further studies are needed to characterize and compare the effects of PPP
and PRP on bone healing in vivo.
en_US
Patrocinador
dc.description.sponsorship
National Fund for Scientific and Technological Development of the
Chilean Government (FONDECYT)
11121294
1130618
Financing Fund Research Centers in Priority Areas of the Chilean
Government (FONDAP)
15090007