Chilean IPNV isolates: Robustness analysis of PCR detection
Author
dc.contributor.author
Jorquera, Esteban
Author
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Morales, Paz
Author
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Tapia, David
Author
dc.contributor.author
Torres, Pamela
Author
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Eissler, Yoanna
Author
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Espinoza, Juan C.
Author
dc.contributor.author
Conejeros, Pablo
Author
dc.contributor.author
Kuznar, Juan
Admission date
dc.date.accessioned
2016-11-03T14:25:57Z
Available date
dc.date.available
2016-11-03T14:25:57Z
Publication date
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2016
Cita de ítem
dc.identifier.citation
Electronic Journal of Biotechnology 20 (2016) 28–32
es_ES
Identifier
dc.identifier.other
10.1016/j.ejbt.2016.01.001
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/141109
Abstract
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Background: The genomes of several infectious pancreatic necrosis viruses (IPNVs) isolated in Chile were sequenced with a single amplification approach for both segments A and B. The resulting sequences were then used to determine the conservation of the primer-binding regions used in polymerase chain reaction (PCR)-based diagnostic methods proposed in the literature. Thus, the robustness of each technique was studied, particularly the eventual effect of further mutations within the primer-binding sites.
Results: On analysis, most methods currently used to detect Chilean IPNV varieties were deemed adequate. However, the primers were designed to be genogroup specific, implying that most detection methods pose some risk of detecting all strains prevalent in the country, due to the coexistence of genogroups 1 and 5.
Conclusions: Negative resultsmust be interpreted carefully given the high genomic variability of IPNVs. Detection techniques (quantitative reverse transcription (qRT)-PCR) based on degenerate primers can be used to minimize the possibilities of false-negative detections.
es_ES
Patrocinador
dc.description.sponsorship
Servicio Nacional de Pesca, Sernapesca
1090
Subsecretaria de Pesca y Acuicultura, Subpesca
1548
2013-32-17
CONICYT
FP140010