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Authordc.contributor.authorBodaleo Torres, Felipe 
Authordc.contributor.authorMontenegro Venegas, Carolina 
Authordc.contributor.authorHenríquez, Daniel 
Authordc.contributor.authorCourt, Felipe 
Authordc.contributor.authorGonzález Billault, Christian 
Cita de ítemdc.identifier.citationScientific Reports 6:30069es_ES
Abstractdc.description.abstractMicrotubule-associated protein 1B (MAP1B) is expressed predominantly during the early stages of development of the nervous system, where it regulates processes such as axonal guidance and elongation. Nevertheless, MAP1B expression in the brain persists in adult stages, where it participates in the regulation of the structure and physiology of dendritic spines in glutamatergic synapses. Moreover, MAP1B expression is also found in presynaptic synaptosomal preparations. In this work, we describe a presynaptic phenotype in mature neurons derived from MAP1B knockout (MAP1B KO) mice. Mature neurons express MAP1B, and its deficiency does not alter the expression levels of a subgroup of other synaptic proteins. MAP1B KO neurons display a decrease in the density of presynaptic and postsynaptic terminals, which involves a reduction in the density of synaptic contacts, and an increased proportion of orphan presynaptic terminals. Accordingly, MAP1B KO neurons present altered synaptic vesicle fusion events, as shown by FM4-64 release assay, and a decrease in the density of both synaptic vesicles and dense core vesicles at presynaptic terminals. Finally, an increased proportion of excitatory immature symmetrical synaptic contacts in MAP1B KO neurons was detected. Altogether these results suggest a novel role for MAP1B in presynaptic structure and physiology regulation in vitroes_ES
Patrocinadordc.description.sponsorshipFondecyt 1140325 FONDAP Geroscience Center for Brain Health and Metabolism, Chile 15150012es_ES
Publisherdc.publisherNature Publishing Groupes_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.uri*
Sourcedc.sourceScientific Reportses_ES
Keywordsdc.subjectAmpa receptor endocytosises_ES
Keywordsdc.subjectPostsynaptic densitieses_ES
Keywordsdc.subjectSynaptic plasticityes_ES
Keywordsdc.subjectTransport vesicleses_ES
Keywordsdc.subjectProteomic analysises_ES
Keywordsdc.subjectGabaergic neuronses_ES
Keywordsdc.subjectDendritic spineses_ES
Títulodc.titleMicrotubule-associated protein 1B (MAP1B)-deficient neurons show structural presynaptic deficiencies in vitro and altered presynaptic physiologyes_ES
Document typedc.typeArtículo de revista
Indexationuchile.indexArtículo de publicación ISIes_ES

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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile