Target genes of Dpp/BMP signaling pathway revealed by transcriptome profiling in the early D. melanogaster embryo
Author
dc.contributor.author
Domínguez, Calixto
Author
dc.contributor.author
Zúñiga, Alejandro
Author
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Hanna, Patricia
Author
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Hödar Quiroga, Christian
Author
dc.contributor.author
González, Mauricio
Author
dc.contributor.author
Cambiazo Ayala, Verónica
Admission date
dc.date.accessioned
2017-01-16T12:38:50Z
Available date
dc.date.available
2017-01-16T12:38:50Z
Publication date
dc.date.issued
2016
Cita de ítem
dc.identifier.citation
Gene. 591 (2016) 191–200
es_ES
Identifier
dc.identifier.other
10.1016/j.gene.2016.07.015
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/142448
Abstract
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In the early Drosophilamelanogaster embryo, the gene regulatory network controlled by Dpp signaling is involved
in the subdivision of dorsal ectoderm into the presumptive dorsal epidermis and amnioserosa. In this work, we
aimed to identify newDpp downstreamtargets involved in dorsal ectodermpatterning.We used oligonucleotide
D.melanogaster microarrays to identify the set of genes that are differential expressed betweenwild type embryos
and embryos that overexpress Dpp (nos-Gal4NUAS-dpp) during early stages ofembryo development. By using
this approach, weidentified 358 geneswhose relative abundance significantly increased in response toDpp overexpression.
Among them, we found the entire set of known Dpp target genes that function in dorsal ectoderm
patterning (zen, doc, hnt, pnr, ush, tup, and others) in addition to several up-regulated genes of unknown functions.
Spatial expression pattern of up-regulated genes in response to Dpp overexpression aswell as their opposing
transcriptional responses to Dpp loss- and gain-of-function indicated that they are new candidate target
genes of Dpp signaling pathway. We further analyse one of the candidate genes, CG13653, which is expressed
at the dorsal-most cells of the embryo during a restricted period of time. CG13653 orthologs were not detected
in basal lineages of Dipterans, which unlike D. melanogaster develop two extra-embryonic membranes, amnion
and serosa. We characterized the enhancer region of CG13653 and revealed that CG13653 is directly regulated
by Dpp signaling pathway.