Author | dc.contributor.author | Schiffer, Jamie M. | |
Author | dc.contributor.author | Malmstrom, Robert D. | |
Author | dc.contributor.author | Parnell, Jonathan | |
Author | dc.contributor.author | Ramírez Sarmiento, César | |
Author | dc.contributor.author | Reyes, Javiera | |
Author | dc.contributor.author | Amaro, Rommie E. | |
Author | dc.contributor.author | Komives, Elizabeth A. | |
Admission date | dc.date.accessioned | 2017-03-02T15:17:30Z | |
Available date | dc.date.available | 2017-03-02T15:17:30Z | |
Publication date | dc.date.issued | 2016 | |
Cita de ítem | dc.identifier.citation | Structure. Volumen: 24 Número: 8 Páginas: 1248-1256 | es_ES |
Identifier | dc.identifier.other | 10.1016/j.str.2016.05.016 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/142977 | |
Abstract | dc.description.abstract | Cullin-RING E3 ligases (CRLs) are elongated and bowed protein complexes that transfer ubiquitin over 60 angstrom to proteins targeted for proteasome degradation. One such CRL contains the ankyrin repeat and SOCS box protein 9 (ASB9), which binds to and partially inhibits creatine kinase (CK). While current models for the ASB9-CK complex contain some known interface residues, the overall structure and precise interface of the ASB9-CK complex remains unknown. Through an integrative modeling approach, we report a third-generation model that reveals precisely the interface interactions and also fits the shape of the ASB9-CK complex as determined by small-angle X-ray scattering. We constructed an atomic model for the entire CK-targeting CRL to uncover dominant modes of motion that could permit ubiquitin transfer. Remarkably, only the correctly docked CK-containing E3 ligase and not incorrectly docked structures permitted close approach of ubiquitin to the CK substrate. | es_ES |
Lenguage | dc.language.iso | en | es_ES |
Source | dc.source | Structure | es_ES |
Keywords | dc.subject | profile | es_ES |
Keywords | dc.subject | insights | es_ES |
Keywords | dc.subject | recognition | es_ES |
Keywords | dc.subject | docking | es_ES |
Keywords | dc.subject | simulation;prediction | es_ES |
Keywords | dc.subject | crystal-structure | es_ES |
Keywords | dc.subject | cullin-ring ligases | es_ES |
Keywords | dc.subject | state analog complex | es_ES |
Keywords | dc.subject | muscle creatine-kinase | es_ES |
Título | dc.title | Model of the Ankyrin and SOCS Box Protein, ASB9, E3 Ligase Reveals a Mechanism for Dynamic Ubiquitin Transfer | es_ES |
Document type | dc.type | Artículo de revista | |
dcterms.accessRights | dcterms.accessRights | Acceso a solo metadatos | es_ES |
Cataloguer | uchile.catalogador | C. R. B. | es_ES |
Indexation | uchile.index | Artículo de publicación ISI | es_ES |