Show simple item record

Authordc.contributor.authorGonzález Mañan, Daniel 
Authordc.contributor.authorD'Espessailles Tapia, Amanda 
Authordc.contributor.authorDossi Muñoz, Camila 
Authordc.contributor.authorSan Martín, Marcela 
Authordc.contributor.authorMancilla, Rodrigo A. 
Authordc.contributor.authorTapia Opazo, Gladys 
Admission datedc.date.accessioned2018-03-26T13:14:23Z
Available datedc.date.available2018-03-26T13:14:23Z
Publication datedc.date.issued2017-04
Cita de ítemdc.identifier.citationJournal of Nutrition Volumen: 147 Número: 4 Páginas: 579-588 (2017)es_ES
Identifierdc.identifier.other10.3945/jn.116.243261
Identifierdc.identifier.urihttp://repositorio.uchile.cl/handle/2250/146988
Abstractdc.description.abstractBackground: Rosa mosqueta (RM) oil is characterized by high concentrations of antioxidants and alpha-linolenic acid (ALA; 18: 3n-3). We have previously demonstrated in male C57BL/6J mice that RM decreases hepatic steatosis, a condition strongly associated with oxidative stress and inflammation. Objective: We studied the molecular mechanisms that underlie the role of RM in preventing high-fat diet (HFD)-induced oxidative stress and inflammation. Methods: Male C57BL/6J mice aged 28 d and weighing 12-14 g were divided into the following groups and fed for 12 wk: control diet (CD; 10% fat, 20% protein, and 70% carbohydrates); CD + RM (1.94 mg ALA . g body weight(-1) . d(-1) administered by oral gavage); HFD (60% fat, 20% protein, and 20% carbohydrates); and HFD + RM. General parameters (body weight, visceral fat, and histology); glucose metabolism [homeostasis model assessment and blood glucose area under the curve (AUC)]; oxidative stress [hepatic nuclear factor (erythroid-derived 2)-like-2 (NRF2) and heme oxygenase 1 (HO-1) concentrations]; and inflammation [hepatic peroxisome proliferator-activated receptor alpha (PPAR-alpha) and acylcoenzyme A oxidase 1 (ACOX1) concentrations, blood tumor necrosis factor alpha (TNF-alpha) and interleukin 1 beta (IL-1 beta) concentrations, and Tnfa and Il1b mRNA expression in liver and visceral adipose tissue] were evaluated. Results: In the HFD + RM mice, the final body weight (24.8 +/- 1.1 g) was 19% lower than in the HFD mice (30.+/- 6 2.8 g) (P < 0.05). Visceral fat was 34% lower in the HFD + RM mice than in the HFD mice (P < 0.05). The blood glucose AUC was 29% lower and Tnfa and Il1b expression levels were 47% and 59% lower, respectively, in the HFD + RM mice than in the HFD mice (P < 0.05). HFD + RM mice had 40% less hepatic steatosis (P < 0.05) and lower upregulation of PPAR-alpha (33%), ACOX1 (50%), NRF2 (39%), and HO-1 (68%) protein concentrations than did the HFD mice (P < 0.05). Conclusions: Our findings suggest that RM supplementation prevents the obese phenotype observed in HFD-fed mice by downregulating inflammatory cytokine expression and secretion and stimulating hepatic antioxidant and fatty acid oxidation markers.es_ES
Patrocinadordc.description.sponsorshipNational Fund for Scientific and Technological Development 1140547es_ES
Lenguagedc.language.isoenes_ES
Publisherdc.publisherAmerican Society for Nutritiones_ES
Sourcedc.sourceJournal of Nutritiones_ES
Keywordsdc.subjectRosa mosquetaes_ES
Keywordsdc.subjectInflammationes_ES
Keywordsdc.subjectOxidative stresses_ES
Keywordsdc.subjectPPAR-alphaes_ES
Keywordsdc.subjectNRF2es_ES
Títulodc.titleRosa mosqueta oil prevents oxidative stress and inflammation through the upregulation of PPAR-alpha and NRF2 in C57BL/6J mice fed a high-fat dietes_ES
Document typedc.typeArtículo de revistaes_ES
dcterms.accessRightsdcterms.accessRightsAcceso a solo metadatoses_ES
Catalogueruchile.catalogadorpgves_ES
Indexationuchile.indexArtículo de publicación ISIes_ES


Files in this item

Icon

This item appears in the following Collection(s)

Show simple item record