Rosa mosqueta oil prevents oxidative stress and inflammation through the upregulation of PPAR-alpha and NRF2 in C57BL/6J mice fed a high-fat diet
Access noteAcceso a solo metadatos
MetadataShow full item record
Background: Rosa mosqueta (RM) oil is characterized by high concentrations of antioxidants and alpha-linolenic acid (ALA; 18: 3n-3). We have previously demonstrated in male C57BL/6J mice that RM decreases hepatic steatosis, a condition strongly associated with oxidative stress and inflammation. Objective: We studied the molecular mechanisms that underlie the role of RM in preventing high-fat diet (HFD)-induced oxidative stress and inflammation. Methods: Male C57BL/6J mice aged 28 d and weighing 12-14 g were divided into the following groups and fed for 12 wk: control diet (CD; 10% fat, 20% protein, and 70% carbohydrates); CD + RM (1.94 mg ALA . g body weight(-1) . d(-1) administered by oral gavage); HFD (60% fat, 20% protein, and 20% carbohydrates); and HFD + RM. General parameters (body weight, visceral fat, and histology); glucose metabolism [homeostasis model assessment and blood glucose area under the curve (AUC)]; oxidative stress [hepatic nuclear factor (erythroid-derived 2)-like-2 (NRF2) and heme oxygenase 1 (HO-1) concentrations]; and inflammation [hepatic peroxisome proliferator-activated receptor alpha (PPAR-alpha) and acylcoenzyme A oxidase 1 (ACOX1) concentrations, blood tumor necrosis factor alpha (TNF-alpha) and interleukin 1 beta (IL-1 beta) concentrations, and Tnfa and Il1b mRNA expression in liver and visceral adipose tissue] were evaluated. Results: In the HFD + RM mice, the final body weight (24.8 +/- 1.1 g) was 19% lower than in the HFD mice (30.+/- 6 2.8 g) (P < 0.05). Visceral fat was 34% lower in the HFD + RM mice than in the HFD mice (P < 0.05). The blood glucose AUC was 29% lower and Tnfa and Il1b expression levels were 47% and 59% lower, respectively, in the HFD + RM mice than in the HFD mice (P < 0.05). HFD + RM mice had 40% less hepatic steatosis (P < 0.05) and lower upregulation of PPAR-alpha (33%), ACOX1 (50%), NRF2 (39%), and HO-1 (68%) protein concentrations than did the HFD mice (P < 0.05). Conclusions: Our findings suggest that RM supplementation prevents the obese phenotype observed in HFD-fed mice by downregulating inflammatory cytokine expression and secretion and stimulating hepatic antioxidant and fatty acid oxidation markers.
National Fund for Scientific and Technological Development 1140547
Artículo de publicación ISI
Quote ItemJournal of Nutrition Volumen: 147 Número: 4 Páginas: 579-588 (2017)