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Authordc.contributor.authorVillar, Pablo 
Authordc.contributor.authorDelgado Arriagada, Ricardo 
Authordc.contributor.authorVergara Montecinos, Cecilia 
Authordc.contributor.authorReyes, Juan G. 
Authordc.contributor.authorBacigalupo Vicuña, Juan 
Cita de ítemdc.identifier.citationThe Journal of Neuroscience, June 7, 2017 • 37(23):5736 –5743es_ES
Abstractdc.description.abstractThe mechanisms that power the physiological events occurring in cilia, flagella, and microvilli are of fundamental importance for the functions of these important and ubicuous organelles. The olfactory epithelium is mostly populated by ciliated olfactory sensory neurons (OSNs) and surrounding sustentacular cells (SCs) with apical microvilli. The only OSN dendrite extends to the surface forming a knob projecting several chemosensory cilia of similar to 50 x 0.2 mu m, devoid of inner membranes embedded in a mucus layer. Uponodorant binding, odor receptors couple to G-protein activating adenylyl cyclase, producing cAMP. cAMP opens cyclic nucleotide-gated channels allowing a Ca2+ influx that opens Ca2+ activated Cl- channels, generating the receptor potential. Many enzymes are activated in chemotransduction to hydrolyze ATP. The knob contains approximately two mitochondria; assuming that the cilia ATP is 1 mM and diffuses along it at similar to 10 mu m in 500 ms, ATP from the knob mitochondria may not fulfill the demands of transduction over the full length of the cilium, which suggests an additional ATP source. We measured millimolar glucose in rat mucus; we detected glucose transporter GLUT3 in rat and toad (Caudiverbera caudiverbera) OSN cilia, SC microvilli, and glycolytic enzymes in rat cilia. We also found that the cilia and knob can incorporate and accumulate 2-deoxyglucose (glucose analog), but not when blocking GLUT. Glucose removal and the inhibition of glycolysis or oxidative phospholylation impaired the odor response. This evidence strongly suggests that glycolysis in the cilia and knob oxidative phosphorylation together fuel chemotransduction.es_ES
Patrocinadordc.description.sponsorshipFondo Nacional de Ciencia y Tecnología 1140520es_ES
Publisherdc.publisherSoc Neurosciencees_ES
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile*
Link to Licensedc.rights.uri*
Sourcedc.sourceThe Journal of Neurosciencees_ES
Keywordsdc.subjectGlucose transportes_ES
Keywordsdc.subjectOlfactory ciliaes_ES
Keywordsdc.subjectOxidative phosphorylationes_ES
Títulodc.titleEnergy requirements of odor transduction in the chemosensory cilia of olfactory sensory neurons rely on oxidative phosphorylation and glycolytic processing of extracellular glucosees_ES
Document typedc.typeArtículo de revista
Indexationuchile.indexArtículo de publicación ISIes_ES

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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile