Intestinal microbiota of white shrimp penaeus vannamei under intensive cultivation conditions in Ecuador
Author
dc.contributor.author
Gainza, Oreste
Author
dc.contributor.author
Jorquera Ramírez, Carolina
Author
dc.contributor.author
Salinas Ramos, Alfredo
Author
dc.contributor.author
Romero Ormazábal, Jaime
Admission date
dc.date.accessioned
2018-07-17T16:33:36Z
Available date
dc.date.available
2018-07-17T16:33:36Z
Publication date
dc.date.issued
2018
Cita de ítem
dc.identifier.citation
Microb Ecol (2018) 75: 562–568
es_ES
Identifier
dc.identifier.other
10.1007/s00248-017-1066-z
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/149926
Abstract
dc.description.abstract
The goal of the study was to characterize the intestinal tract bacterial microbiota composition of Penaeus vannamei in intensive commercial ponds in Ecuador, comparing two shrimp-farming phases: nursery and harvest. Bacterial microbiota was examined by sequencing amplicons V2-V3 of the 16S rRNA using Ion Torrent technology. Archaea sequences were detected in both phases. Sequence analyses revealed quantitative and qualitative differences between the nursery phase and the harvest phase in shrimp intestinal microbiota composition. The main differences were observed at the phylum level during the nursery phase, and the prevailing phyla were CKC4 (37.3%), Proteobacteria (29.8%), Actinobacteria (11.6%), and Firmicutes (10.1%). In the harvest phase, the prevailing phyla were Proteobacteria (28.4%), Chloroflexi (19.9%), and Actinobacteria (15.1%). At the genus level, microbiota from the nursery phase showed greater relative abundances of CKC4 uncultured bacterium (37%) and Escherichia-Shigella (18%). On the contrary, in the microbiota of harvested shrimp, the prevailing genera were uncultured Caldilinea (19%) and Alphaproteobacteria with no other assigned rate (10%). The analysis of similarity ANOSIM test (beta diversity) indicated significant differences between the shrimp microbiota for these two farming phases. Similarly, alfa-diversity analysis (Chao1) indicated that the microbiota at harvest was far more diverse than the microbiota during the nursery phase, which showed a homogeneous composition. These results suggest that shrimp microbiota diversify their composition during intensive farming. The present work offers the most detailed description of the microbiota of P. vannamei under commercial production conditions to date.