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Authordc.contributor.authorRivera, Dacil 
Authordc.contributor.authorToledo, Viviana 
Authordc.contributor.authorDi Pillo, Francisca 
Authordc.contributor.authorDueñas, Fernando 
Authordc.contributor.authorTardone, Rodolfo 
Authordc.contributor.authorHamilton-West Miranda, Christopher 
Authordc.contributor.authorVongkamjan, Kitiya 
Authordc.contributor.authorWiedmann, Martin 
Authordc.contributor.authorMoreno Switt, Andrea I. 
Admission datedc.date.accessioned2018-08-01T16:40:38Z
Available datedc.date.available2018-08-01T16:40:38Z
Publication datedc.date.issued2018
Cita de ítemdc.identifier.citationJournal of Food Protection 81(2) : 272-278es_ES
Identifierdc.identifier.other10.4315/0362-028X.JFP-17-075
Identifierdc.identifier.urihttps://repositorio.uchile.cl/handle/2250/150533
Abstractdc.description.abstractThe genus Salmonella has more than 2,600 serovars, and this trait is important when considering interventions for Salmonella control. Bacteriophages that are used for biocontrol must have an exclusively lytic cycle and the ability to lyse several Salmonella serovars under a wide range of environmental conditions. Salmonella phages were isolated and characterized from 34 backyard production systems (BPSs) with a history of Salmonella infections. BPSs were visited once, and cloacal or fecal samples were processed for phage isolation. Four hosts, Salmonella serovars Enteritidis, Heidelberg, Infantis, and Typhimurium, were used for phage isolation. The host range of the phages was later characterized with a panel of 23 Salmonella serovars (serovar diversity set) and 31 isolates obtained from the same farms (native set). Genetic relatedness for 10 phages with a wide host range was characterized by restriction fragment length polymorphism, and phages clustered based on the host range. We purified 63 phages, and 36 phage isolates were obtained on Salmonella Enteritidis, 16 on Salmonella Heidelberg, and 11 on Salmonella Infantis. Phages were classified in three clusters: (i) phages with a wide host range (cluster I), (ii) phages that lysed the most susceptible Salmonella serovars (serogroup D) and other isolates (cluster II), and (iii) phages that lysed only isolates of serogroup D (cluster III). The most susceptible Salmonella serovars were Enteritidis, Javiana, and Dublin. Seven of 34 farms yielded phages with a wide host range, and these phages had low levels of genetic relatedness. Our study showed an adaptation of the phages in the sampled BPSs to serogroup D Salmonella isolates and indicated that isolation of Salmonella phages with wide host range differs by farm. A better understanding of the factors driving the Salmonella phage host range could be useful when designing risk-based sampling strategies to obtain phages with a wide lytic host range for biocontrol purposes.es_ES
Patrocinadordc.description.sponsorshipFONDECYT 11140108 11121389es_ES
Lenguagedc.language.isoenes_ES
Publisherdc.publisherInternational Association Food Protectiones_ES
Sourcedc.sourceJournal of Food Protectiones_ES
Keywordsdc.subjectChicken phagees_ES
Keywordsdc.subjectPhage host rangees_ES
Keywordsdc.subjectPig phagees_ES
Keywordsdc.subjectSalmonella phagees_ES
Títulodc.titleBackyard farms represent a source of wide host range salmonella phages that lysed the most common salmonella serovarses_ES
Document typedc.typeArtículo de revista
dcterms.accessRightsdcterms.accessRightsAcceso a solo metadatoses_ES
Catalogueruchile.catalogadortjnes_ES
Indexationuchile.indexArtículo de publicación ISIes_ES


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