Diversity and geographical distribution of flavobacterium psychrophilum Isolates and their phages: patterns of dusceptibility to phage infection and phage host range
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2014Metadata
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Castillo, Daniel
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Diversity and geographical distribution of flavobacterium psychrophilum Isolates and their phages: patterns of dusceptibility to phage infection and phage host range
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Abstract
Flavobacterium psychrophilum is an important fish
pathogen worldwide that causes cold water disease (CWD) or
rainbow trout fry syndrome (RTFS). Phage therapy has been
suggested as an alternative method for the control of this
pathogen in aquaculture. However, effective use of bacteriophages
in disease control requires detailed knowledge about the
diversity and dynamics of host susceptibility to phage infection.
For this reason, we examined the genetic diversity of 49
F. psychrophilum strains isolated in three different areas
(Chile, Denmark, and USA) through direct genome restriction
enzyme analysis (DGREA) and their susceptibility to 33 bacteriophages
isolated in Chile and Denmark, thus covering large
geographical (>12,000 km) and temporal (>60 years) scales of
isolation. An additional 40 phage-resistant isolates obtained
from culture experiments after exposure to specific phageswere
examined for changes in phage susceptibility against the 33
phages. The F. psychrophilum and phage populations isolated
from Chile and Denmark clustered into geographically distinct
groups with respect to DGREA profile and host range,
respectively. However, cross infection between Chilean phage
isolates and Danish host isolates and vice versa was observed.
Development of resistance to certain bacteriophages led to
susceptibility to other phages suggesting that “enhanced infection”
is potentially an important cost of resistance in
F. psychrophilum, possibly contributing to the observed coexistence
of phage-sensitive F. psychrophilum strains and lytic
phages across local and global scales. Overall, our results
showed that despite the identification of local communities of
phages and hosts, some key properties determining phage
infection patterns seem to be globally distributed.
General note
Artículo de publicación ISI
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This work was partially supported by Grant
INNOVA 07CN13PPT-09 of CORFO-Chile, by a grant from The Danish
Council for Independent Research (FNU-09-072829) and The Danish
Directorate for Food, Fisheries and Agri Business (ProAqua, project # 09-
072829) to MMand by the EU-IRSES-funded project AQUAPHAGE to
MM and RE.
Identifier
URI: https://repositorio.uchile.cl/handle/2250/124134
DOI: DOI: 10.1007/s00248-014-0375-8
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Microb Ecol (2014) 67:748–757
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