Molecular characterization of totiviruses in Xanthophyllomyces dendrorhous
Author
dc.contributor.author
Baeza Cancino, Marcelo
Author
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Bravo, Natalia
Author
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Sanhueza, Mario
Author
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Flores, Oriana
Author
dc.contributor.author
Villarreal, Pablo
Author
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Cifuentes Guzmán, Víctor
Admission date
dc.date.accessioned
2018-12-20T14:06:16Z
Available date
dc.date.available
2018-12-20T14:06:16Z
Publication date
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2012
Cita de ítem
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Virology Journal, Volumen 9,
Identifier
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1743422X
Identifier
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10.1186/1743-422X-9-140
Identifier
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https://repositorio.uchile.cl/handle/2250/153883
Abstract
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Abstract. Background: Occurrence of extrachromosomal dsRNA elements has been described in the red-yeast Xanthophyllomyces dendrorhous, with numbers and sizes that are highly variable among strains with different geographical origin. The studies concerning to the encapsidation in viral-like particles and dsRNA-curing have suggested that some dsRNAs are helper viruses, while others are satellite viruses. However, the nucleotide sequences and functions of these dsRNAs are still unknown. In this work, the nucleotide sequences of four dsRNAs of the strain UCD 67-385 of X. dendrorhous were determined, and their identities and genome structures are proposed. Based on this molecular data, the dsRNAs of different strains of X. dendrorhous were analyzed. Results: The complete sequences of L1, L2, S1 and S2 dsRNAs of X. dendrorhous UCD 67-385 were determined, finding two sequences for L1 dsRNA (L1A and L1B). Several ORFs were uncovered in both S1 and S2 dsRNAs, but no homologies were found for an