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Author | dc.contributor.author | Cardemil, Emilio | |
Author | dc.contributor.author | Eyzaguirre, Jaime | |
Admission date | dc.date.accessioned | 2018-12-20T14:08:12Z | |
Available date | dc.date.available | 2018-12-20T14:08:12Z | |
Publication date | dc.date.issued | 1979 | |
Cita de ítem | dc.identifier.citation | Archives of Biochemistry and Biophysics, Volumen 192, Issue 2, 2018, Pages 533-538 | |
Identifier | dc.identifier.issn | 10960384 | |
Identifier | dc.identifier.issn | 00039861 | |
Identifier | dc.identifier.other | 10.1016/0003-9861(79)90123-1 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/154132 | |
Abstract | dc.description.abstract | Rabbit muscle pyruvate kinase is inactivated by 2,3-butanedione in borate buffer. The inactivation follows pseudo-first-order kinetics with a calculated second-order rate constant of 4.6 m-1 min-1. The modification can be reversed with almost total recovery of activity by elimination of the butanedione and borate buffer, suggesting that only arginyl groups are modified; this result agrees with the loss of arginine detected by amino acid analysis of the modified enzyme. Using the kinetic data, it was estimated that the reaction of a single butanedione molecule per subunit of the enzyme is enough to completely inactivate the protein. The inactivation is partially prevented by phosphoenolpyruvate in the presence of K+ and Mg2+, but not by the competitive inhibitors lactate and bicarbonate. These findings point to an essential arginyl residue being located near the phosphate binding site of phosphoenolpyruvate. © 1979. | |
Lenguage | dc.language.iso | en | |
Type of license | dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
Link to License | dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
Source | dc.source | Archives of Biochemistry and Biophysics | |
Keywords | dc.subject | Biophysics | |
Keywords | dc.subject | Biochemistry | |
Keywords | dc.subject | Molecular Biology | |
Título | dc.title | Evidence of essential arginyl residues in rabbit muscle pyruvate kinase | |
Document type | dc.type | Artículo de revista | |
Cataloguer | uchile.catalogador | SCOPUS | |
Indexation | uchile.index | Artículo de publicación SCOPUS | |
uchile.cosecha | uchile.cosecha | SI | |
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