Lysozyme binding to poly(4-vinyl-N-alkylpyridinium bromide)
Author
dc.contributor.author
Silva, Rubens A.
Author
dc.contributor.author
Urzúa Acevedo, Marcela
Author
dc.contributor.author
Petri, Denise F.S.
Admission date
dc.date.accessioned
2018-12-20T14:12:14Z
Available date
dc.date.available
2018-12-20T14:12:14Z
Publication date
dc.date.issued
2009
Cita de ítem
dc.identifier.citation
Journal of Colloid and Interface Science, Volumen 330, Issue 2, 2018, Pages 310-316
Identifier
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00219797
Identifier
dc.identifier.other
10.1016/j.jcis.2008.10.087
Identifier
dc.identifier.uri
https://repositorio.uchile.cl/handle/2250/154688
Abstract
dc.description.abstract
The adsorption behavior of polycations at ionic strengths (I) ranging from 0.001 to 0.1 onto silicon wafers was studied by means of ellipsometry, contact angle measurements and atomic force microscopy (AFM). Polycations chosen were bromide salts of poly(4-vinylpyridine) N-alkyl quaternized with linear aliphatic chains of 2 and 5 carbon atoms, QPVP-C2 and QPVP-C5, respectively. Under I = 0.001 the reduction of screening effects led to low adsorbed amounts of QPVP-C2 or QPVP-C5 (1.0 ± 0.1 mg / m2), arising from the adsorption of extended chains. Upon increasing I to 0.1, screening effects led to conformational changes of polyelectrolyte chains in solution and to higher adsorbed amount values (1.9 ± 0.2 mg / m2). Advancing contact angle θa measurements performed with water drops onto QPVP-C2 and QPVP-C5 adsorbed layers varied from (45 ± 2) ° to (50 ± 5) °, evidencing the exposure of both hydrophobic alkyl groups and charged moieties. The adsorption of lysozyme (LYZ) molecules to QPVP-C5 l