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Authordc.contributor.authorSoto-Gamboa, Mauricio 
Authordc.contributor.authorGonzalez, Sandra 
Authordc.contributor.authorHayes, Loren D. 
Authordc.contributor.authorEbensperger, Luis A. 
Cita de ítemdc.identifier.citationJournal of Experimental Zoology Part A: Ecological Genetics and Physiology, Volumen 311, Issue 7, 2018, Pages 496-503
Abstractdc.description.abstractDetermination of fecal steroid metabolites is a noninvasive technique that characterizes the physiological state of organisms without the physiological and psychological stress of handling. Although this technique has many applications in the study of wildlife and/or captive animals without the necessity of capturing individuals, it requires a species-specific validation before use. A complete validation includes an analytical and a physiological one. In the latter changes in fecal hormone metabolites are induced by previous manipulations of the respective plasma hormones. Here we validated a method for measuring fecal cortisol metabolites (FCM) in the hystricomorph rodent Octodon degus. We extracted feces with 80% ethanol and quantified steroids using a commercial available cortisol radioimmunoassay. We first compared baseline levels of blood cortisol and FCM, and then performed a challenge test with adrenocorticotropic hormone (ACTH) to demonstrate that FCM accurately reflect adrenoc
Type of licensedc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
Link to Licensedc.rights.uri
Sourcedc.sourceJournal of Experimental Zoology Part A: Ecological Genetics and Physiology
Keywordsdc.subjectAnimal Science and Zoology
Keywordsdc.subjectEcology, Evolution, Behavior and Systematics
Keywordsdc.subjectMolecular Biology
Títulodc.titleValidation of a radioimmunoassay for measuring fecal cortisol metabolites in the hystricomorph rodent, octodon degus
Document typedc.typeArtículo de revista
Indexationuchile.indexArtículo de publicación SCOPUS

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Attribution-NonCommercial-NoDerivs 3.0 Chile
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-NoDerivs 3.0 Chile