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Author | dc.contributor.author | Acevedo, Mónica L. | |
Author | dc.contributor.author | Arbildúa, José Jaime | |
Author | dc.contributor.author | Monasterio Opazo, Octavio | |
Author | dc.contributor.author | Toledo, Héctor | |
Author | dc.contributor.author | León, Oscar | |
Admission date | dc.date.accessioned | 2018-12-20T14:12:43Z | |
Available date | dc.date.available | 2018-12-20T14:12:43Z | |
Publication date | dc.date.issued | 2010 | |
Cita de ítem | dc.identifier.citation | Archives of Biochemistry and Biophysics, Volumen 495, Issue 1, 2018, Pages 28-34 | |
Identifier | dc.identifier.issn | 00039861 | |
Identifier | dc.identifier.issn | 10960384 | |
Identifier | dc.identifier.other | 10.1016/j.abb.2009.12.018 | |
Identifier | dc.identifier.uri | https://repositorio.uchile.cl/handle/2250/154778 | |
Abstract | dc.description.abstract | X-ray diffraction data on a few retroviral integrases show a flexible loop near the active site. By sequence alignment, the peptide region 207-218 of Mo-MLV IN appears to correspond to this flexible loop. In this study, residues H208, Y211, R212, Q214, S215 and S216 of Mo-MLV IN were mutated to determine their role on enzyme activity. We found that Y211A, R212A, R212K and Q214A decreased integration activity, while disintegration and 3′-processing were not significantly affected. By contrast H208A was completely inactive in all the assays. The core domain of Mo-MLV integrase was modeled and the flexibility of the region 207-216 was analyzed. Substitutions with low integration activity showed a lower flexibility than wild type integrase. We propose that the peptide region 207-216 is a flexible loop and that H208, Y211, R212 and Q214 of this loop are involved in the correct assembly of the DNA-integrase complex during integration. © 2009 Elsevier Inc. | |
Lenguage | dc.language.iso | en | |
Type of license | dc.rights | Attribution-NonCommercial-NoDerivs 3.0 Chile | |
Link to License | dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
Source | dc.source | Archives of Biochemistry and Biophysics | |
Keywords | dc.subject | B-factor | |
Keywords | dc.subject | Flexible loop | |
Keywords | dc.subject | Integrase | |
Keywords | dc.subject | Molecular modeling | |
Keywords | dc.subject | Mutagenesis | |
Keywords | dc.subject | Retrovirus | |
Título | dc.title | Role of the 207-218 peptide region of Moloney murine leukemia virus integrase in enzyme catalysis | |
Document type | dc.type | Artículo de revista | |
Cataloguer | uchile.catalogador | SCOPUS | |
Indexation | uchile.index | Artículo de publicación SCOPUS | |
uchile.cosecha | uchile.cosecha | SI | |
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